Proteins were isolated and equal amounts were separated by PAGE and transferred to nitrocellulose membranes (Sigma). After blocking, membranes were incubated with primary antibodies (anti-ABCA1, ab18180; anti-β-actin, ab8229; anti-apoA-I, ab7613 [all from Abcam, Cambridge, UK]; and anti-SRBI, NB400-104 [Novus]) at 4°C over night. Membranes were then incubated with the appropriate HRP-coupled secondary antibodies followed by detection using the Super Signal chemiluminescence system (Thermo Scientific, Rockford, IL) and a Chemilmager 4440 (Biozym, Oldendorf, Germany).
Chemilmager 4440
Manufactured by Biozym
Sourced in Germany
The Chemilmager 4440 is a high-performance imaging system designed for the detection and analysis of chemiluminescent signals. It features a sensitive CCD camera, adjustable imaging parameters, and specialized software for data acquisition and analysis.
Automatically generated - may contain errors
Lab products found in correlation
Nitrocellulose membrane, by Merck Group (2 mentions)
Supersignal chemiluminescence system, by Thermo Fisher Scientific (2 mentions)
Ab8229, by Abcam (1 mentions)
Ab7613, by Abcam (1 mentions)
Ab18180, by Abcam (1 mentions)
Nb400 104, by Novus Biologicals (1 mentions)
Anti cd36, by Cayman Chemical (1 mentions)
Anti sr bi, by BD (1 mentions)
Anti β actin, by Abcam (1 mentions)
2 protocols using chemilmager 4440
1
Western Blot Protein Detection
2
Western Blot Analysis of Membrane Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Proteins were isolated and equal amounts were separated by SDS-PAGE and transferred to nitrocellulose membranes (Sigma). After blocking, membranes were incubated with primary antibodies (anti-SR-BI, BD Biosciences 610882; anti-β-actin, Abcam ab8229; anti-CD36, Cayman 100011) at 4°C over-night. Membranes were then incubated with the appropriate horseradish peroxidase-coupled secondary antibodies followed by detection using the Super Signal chemiluminescence system (Thermo Scientific) and a Chemilmager 4440 (Biozym, Oldendorf, Germany).
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