Anti foxp3 alexa fluor 488 clone mf23

Manufactured by BD

Anti-FoxP3 Alexa Fluor 488 (clone MF23) is a fluorescent-labeled antibody that binds to the FoxP3 protein. FoxP3 is a transcription factor that is expressed in regulatory T cells. The Alexa Fluor 488 dye is used to label the antibody, allowing for the detection and analysis of FoxP3-expressing cells by flow cytometry.

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Lab products found in correlation

Anti cd3 pacific blue clone 17a2, by BioLegend (2 mentions) Il 2 fitc clone jes6 5h4, by BD (2 mentions) Live dead fixable aqua dead cell stain kit, by Thermo Fisher Scientific (2 mentions) Anti cd44 pe cy7 clone im7, by BioLegend (1 mentions) Anti ifnγ alexafluor 700, by BD (1 mentions) Anti cd11b alexa fluor 700 clone m1 70, by BD (1 mentions) Anti cd4 percp clone rm4 5, by BD (1 mentions) Tnf α pe cy7, by BD (1 mentions) Anti cd8 pe, by BD (1 mentions) Anti cd45 pe cy5 clone 30 f11, by BioLegend (1 mentions) Anti ly 6g pe cy7 clone 1a8, by BioLegend (1 mentions) Anti ly 6c apc cy7 clone al 21, by BioLegend (1 mentions) Cd11c fitc clone hl3, by BD (1 mentions) Anti granzyme b qa16a02, by BioLegend (1 mentions) Cd11b alexa fluor 700, by BioLegend (1 mentions)

2 protocols using anti foxp3 alexa fluor 488 clone mf23

Comprehensive Immune Cell Profiling

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The following Abs were used for cell surface staining: anti-CD3 Pacific Blue (clone 17A2), anti-CD8 PE Texas Red (clone 53.6.7), anti-CD44 PE-Cy7 (clone IM7), anti-B220 PE Texas Red (clone RA3-6B2), anti-NK1.1 FITC (clone PK136), anti-CD69 Alexa Fluor 647 (clone H1-2F3), anti-Ly6C APC-Cy7 (AL-21), anti-Ly6G PE-Cy7 (clone 1A8) (Biolegend, San Diego, CA, USA), anti-CD4 APC e-Fluor 780 (clone GK1.5), anti-CD62L PE-Cy5 (clone MEL-14) (eBiosciences, San Diego, CA, USA), anti-CD45RB PE (clone 16A), anti-CD3 Alexa Fluor 647 (clone 17A2), anti-CD4 PerCP (clone RM4-5), anti-CD8 PE (clone 53-6.7), anti-CD45 PE-Cy5 (clone 30-F11), anti-CD45 PE (clone 30-F11), anti-CD11c FITC (clone HL3) and anti-CD11b Alexa Fluor 700 (clone M1/70) (BD Biosciences, San José, CA, USA). The abs for intracellular staining included anti-FoxP3 Alexa Fluor 488 (clone MF23), anti-IFNγ Alexa Fluor 700 (clone XMG1.2), TNFα PE-Cy7 (clone MP6-XT22), IL-2 FITC (clone JES6-5H4) (BD Biosciences) and anti-CTLA-4 PE (clone UC10-4F10-11) (Biolegend). A LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Invitrogen Molecular Probes, Eugene, OR) was used for dead cell exclusion.

Lasso P., Gomez-Cadena A., Urueña C., Donda A., Martinez-Usatorre A., Barreto A., Romero P, & Fiorentino S. (2018). Prophylactic vs. Therapeutic Treatment With P2Et Polyphenol-Rich Extract Has Opposite Effects on Tumor Growth. Frontiers in Oncology, 8, 356.

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Multiparametric Flow Cytometry Analysis

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The Abs used for cell-surface staining included: anti-CD3 Pacific Blue (clone 17A2), anti-CD8 PE Dazzle 594 (clone 53.6.7), anti-CD45 PE-Cy5 (clone 30-F11), anti-Ly-6G PE-Cy7 (clone 1A8), anti-Ly-6C APC-Cy7 (clone AL-21), anti-PD-L1 PE (clone 10F.9G2), anti-PD-1 APC (clone 29F-1A12), CD11b Alexa Fluor 700 (clone M1/70), anti-CD4 Brilliant Violet 570 (clone RM4-5), anti-CD44 PE-Cy7 (clone IM7), CD25 APC (clone 3C7) (Biolegend, San Diego, CA, USA), and CD11c FITC (clone HL3) (BD Biosciences, San José, CA, USA). The abs for intracellular staining were anti-FoxP3 Alexa Fluor 488 (clone MF23) (BD Biosciences) and anti-CTLA-4 PE (clone UC10-4F10-11) (Biolegend). A LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Life Technologies, Thermo Scientific, Eugene, OR, USA) was used for dead cell exclusion. The abs used for intracellular cytokines evaluation were anti-IFNγ Alexa Fluor 700 (clone XMG1.2), TNFα PE-Cy7 (clone MP6-XT22), IL-2 FITC (clone JES6-5H4) (BD Biosciences), anti-perforin (clone S16009A), and anti-granzyme B (QA16A02) (Biolegend).

Lasso P., Rojas L., Arévalo C., Urueña C., Murillo N., Barreto A., Costa G.M, & Fiorentino S. (2022). Tillandsia usneoides Extract Decreases the Primary Tumor in a Murine Breast Cancer Model but Not in Melanoma. Cancers, 14(21), 5383.

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