Fenofibrate (Feno) from Fournier Pharma (France); Fluvastatin (Flu) from Beijing Novartis Pharma Ltd; Tetracycline was purchased from AMRESCO (USA); Polyene Phosphatidylcholine (PPC) from Sanofi, Beijing (China); Rosiglitazone (Rosi) from GSK, China; Anti-β-actin antibody, anti-FAS antibody, anti-ACC antibody from Cell Signaling Technology (USA); anti-MTTP antibody, anti-CPT-1 antibody, anti-CD36 antibody from Abcam (USA); anti-SREBP-1c antibody from SANTA (USA); commercial kits for liver triglyceride, ECL reactions from Applygen Technologies Inc.
Anti fas antibody
Manufactured by Cell Signaling Technology
Sourced in China
The Anti-Fas antibody is a laboratory tool used to detect the Fas receptor, also known as CD95 or Apoptosis Antigen 1 (APO-1). The Fas receptor is a member of the tumor necrosis factor (TNF) receptor superfamily and plays a key role in the initiation of apoptosis, or programmed cell death. The Anti-Fas antibody can be used in various research applications, such as flow cytometry, immunohistochemistry, and Western blotting, to identify and analyze Fas receptor expression in different cell types and tissues.
Automatically generated - may contain errors
Lab products found in correlation
Tetracycline, by Avantor (1 mentions)
Anti cd36 antibody, by Abcam (1 mentions)
Rosiglitazone rosi, by GlaxoSmithKline (1 mentions)
Anti acc antibody, by Cell Signaling Technology (1 mentions)
Anti cpt 1 antibody, by Abcam (1 mentions)
Anti β actin antibody, by Cell Signaling Technology (1 mentions)
Tunel reagent, by Elabscience (1 mentions)
Image pro plus 7, by Media Cybernetics (1 mentions)
Horseradish peroxidase hrp conjugated secondary antibody, by Gene Tech (1 mentions)
Fluorescence microscope, by Leica (1 mentions)
Protein a g agarose, by Santa Cruz Biotechnology (1 mentions)
Np 40, by Merck Group (1 mentions)
3 protocols using anti fas antibody
1
Lipid Metabolism Regulation Protocol
2
Histopathological Analysis of Intestinal Injury
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After fixation in 10% formalin, the intestinal tissues were embedded in paraffin wax and cut into 3 μm slices. The slices were then dehydrated with gradient alcohol, cleaned with xylene, and sealed with resin. Hematoxylin and eosin (H&E) staining was performed on the slices. Finally, images were captured using a light microscope (Nikon, Tokyo, Japan) to detect histopathological changes. The severity of intestinal injury was assessed according to Chiu’s scoring system, as previously described [9 (link)].
For immunohistochemistry (IHC), tissue slices were treated with anti-Fas antibody (1:500 dilution; Cell Signaling Technology). The horseradish peroxidase (HRP)-conjugated secondary antibody (Gene Tech, Shanghai, China) was incubated for 30 min at room temperature and used to detect the primary antibody. The images were acquired using a light microscope (Nikon), and Fas staining was quantified using Image-Pro Plus 7 (Media Cybernetics, MD, USA).
Apoptotic cells in the intestinal epithelium sections were detected using TdT-mediated dUTP-biotin nick end-labeling (TUNEL) reagent (Elabscience, Wuhan, China) according to the manufacturer’s instructions. Images were acquired using a fluorescence microscope (Leica Microsystems), and TUNEL-positive cells were quantified using Image-Pro Plus 7 (Media Cybernetics).
For immunohistochemistry (IHC), tissue slices were treated with anti-Fas antibody (1:500 dilution; Cell Signaling Technology). The horseradish peroxidase (HRP)-conjugated secondary antibody (Gene Tech, Shanghai, China) was incubated for 30 min at room temperature and used to detect the primary antibody. The images were acquired using a light microscope (Nikon), and Fas staining was quantified using Image-Pro Plus 7 (Media Cybernetics, MD, USA).
Apoptotic cells in the intestinal epithelium sections were detected using TdT-mediated dUTP-biotin nick end-labeling (TUNEL) reagent (Elabscience, Wuhan, China) according to the manufacturer’s instructions. Images were acquired using a fluorescence microscope (Leica Microsystems), and TUNEL-positive cells were quantified using Image-Pro Plus 7 (Media Cybernetics).
3
Fas-induced Apoptosis Signaling Complex Isolation
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HepG2 cells were incubated with anti-Fas CH11 plus 0.5 mg/ mL actinomycin D for 4 hours at 37 C, then lysed in immunoprecipitation/lysis buffer (150 nmol/L NaCl, 50 mmol/L Tris, pH 7.5, 1.0 mmol/L EDTA, 1.0 mmol/L dithiothreitol, 1% NP-40 (MilliporeSigma, St. Louis, MO), 10 mmol/L bphosphoglycerate, 0.1 mmol/L NaF, 1 mmol/L NaVO 4 , and small peptide inhibitors). Lysates were precleared by incubation with 30 mL of protein Geagarose beads (50% slurry in immunoprecipitation/lysis buffer). The DISC was immunoprecipitated for 12 hours at 4 C using anti-Fas antibody (catalog number 8023; Cell Signaling Technology) and protein A/ G agarose (Santa Cruz Biotechnology, Santa Cruz, CA). After immunoprecipitation, the beads were washed four times with immunoprecipitation/lysis buffer, and the immunoprecipitants were eluted from beads with 2Â Laemmli buffer (2.1% SDS, 26.3% glycerol, 10% 2-mercaptoethanol, 0.01% bromophenol blue, 65.8 mmol/L Tris-HCl, pH 6.8) at 95 C for 5 minutes and subjected to SDS-PAGE, followed by immunoblot analysis.
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