Lipase from porcine pancreas type II (PPL), bovine serum albumin (BSA), tert-butyl alcohol, tributyrin, polyethyleneimine (PEI, average Mn ~ 423), dodecyl aldehyde, and Bradford reagent were purchased from Sigma-Aldrich (St. Louis, MO, USA). Glutaraldehyde solution (25% in H2O) was purchased from Vetec Química Fina (Duque de Caixas, RJ, Brazil). Soluble starch was purchased from Panreac Química (Barcelona, Spain). Anhydrous ethanol (99.8% P.A.) was purchased from Synth (Diadema, SP, Brazil). Soy protein was acquired from local market. Silica magnetic nanoparticles (SMNPs) functionalized with amine groups derived from 3-aminopropyltriethoxysilane (APTS) (136 ± 10 µmol amino/g) were purchased from Kopp Technologies (São Carlos, SP, Brazil).
Dodecyl aldehyde
Manufactured by Merck Group
Sourced in United States
Dodecyl aldehyde is a chemical compound used in laboratory settings. It is a long-chain aliphatic aldehyde with a molecular formula of CH3(CH2)10CHO. Dodecyl aldehyde is a colorless liquid with a characteristic odor.
Automatically generated - may contain errors
Lab products found in correlation
Lipase from porcine pancreas type 2, by Merck Group (1 mentions)
Bradford reagent, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Polyethyleneimine, by Merck Group (1 mentions)
Tert butyl alcohol, by Merck Group (1 mentions)
Tributyrin, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Triton x 100 solution, by Merck Group (1 mentions)
Methyl acrylate, by Merck Group (1 mentions)
Hydrocinnamaldehyde, by Merck Group (1 mentions)
Methyl bromoacetate, by Merck Group (1 mentions)
Resorcinol, by Merck Group (1 mentions)
Ethylendiamine, by Merck Group (1 mentions)
Phosphate buffered saline ph 7.4, by Merck Group (1 mentions)
Hexadecane, by Merck Group (1 mentions)
1 hexadecanol, by Merck Group (1 mentions)
Sodium stearate, by Merck Group (1 mentions)
Chitosan powder, by Merck Group (1 mentions)
4 protocols using dodecyl aldehyde
1
Immobilization of Lipase on Magnetic Nanoparticles
2
Synthesis and Characterization of Ibuprofen Derivatives
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2-(4-Isobutylphenyl) propionic acid (ibuprofen), (98%, Sigma-Aldrich, St. Louis, MO, USA), Resorcinol (99%, Sigma-Aldrich, St. Louis, MO, USA), hydrocinnamaldehyde (90%, Sigma-Aldrich, St. Louis, MO, USA), dodecyl aldehyde (92%, Sigma-Aldrich), hydrochloric acid (36.5–38% Aldrich), ethylendiamine (Sigma-Aldrich, St. Louis, MO, USA), methyl bromoacetate (Sigma-Aldrich, St. Louis, MO, USA), and methyl acrylate (Sigma-Aldrich, St. Louis, MO, USA) were used without additional purification. Triton™ X-100 solution (Sigma-Aldrich, St. Louis, MO, USA), phosphate-buffered saline pH 7.4 (Sigma-Aldrich, St. Louis, MO, USA), and ethanol (Sigma-Aldrich) were used without additional drying. Tetrahydrofuran THF was dried with Na. N,N-Dimethylformamide (DMF, Sigma-Aldrich, St. Louis, MO, USA), and dimethylsulfoxide (DMSO) (99.8%, Sigma-Aldrich, St. Louis, MO, USA).
3
Hydrocarbon Metabolism Analysis of Plastic Degraders
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For analyzing the ability of putative plastic degraders to metabolize hydrocarbons, changes in DCPIP absorbance were monitored while the six strains were cultured with four types of hydrocarbons as carbon sources. Since PE metabolism occurs in the order of alkane, alcohol, aldehyde, fatty acid, followed by lipid metabolism such as β-oxidation, substances that can be utilized by cells were selected. Hexadecane, 1-hexadecanol, dodecyl aldehyde, and sodium stearate (Sigma-Aldrich, Burlington, MA, USA) were used as representatives of alkane, alcohol, aldehyde, and fatty acid, respectively (Table S1 ). These substances have 12–18 carbon chains and can be degraded by lipid metabolism. Experiments were performed with 0.25% (w/v) of each substrate except sodium stearate (0.1%). Bacteria were cultured in LCFBM with DCPIP with each type of hydrocarbon (alkane, alcohol, aldehyde, and fatty acid) as a carbon source. Each cell type was cultured in LB broth for 24 h and inoculated after three washes with 0.9% saline. The DCPIP absorbance of the cultured solution was measured using 1 mL of the centrifuged supernatant. Samples were measured at a wavelength of 600 nm every 24 h for five days.
4
Chitosan-based Nanoparticle Synthesis
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Chitosan powder (low molecular weight, deacetylation degree (DD) ≥ 85%), 2-chloro-N,Ndiethylethylamine hydrochloride (DEAE), dodecyl aldehyde (DDA), acetic acid, sodium acetate, sodium hydroxide, and pyrene were reagent grade and used as received from Sigma Aldrich Chemical Co., Brazil. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), RPMI-1640 cell culture medium and Pur-A-Lyzer™ Mini Dialysis Kit (MWCO 6-8 kDa) were purchased from Sigma-Aldrich GmbH, Germany. Ultrapure MilliQ water was used throughout.
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