Log In Sign up
The largest database of trusted experimental protocols

Pcs 400 030

Manufactured by American Type Culture Collection
Visit Supplier
Sourced in United States

The PCS-400-030 is a laboratory equipment product manufactured by American Type Culture Collection. It is designed to perform specific functions in a laboratory setting. However, without access to detailed technical specifications, a concise and unbiased description of its core function cannot be provided while maintaining objectivity. Therefore, the description for this product is not available.

Automatically generated - may contain errors

Lab products found in correlation

Pcs 400 040, by American Type Culture Collection (4 mentions) Pcs 400 010, by American Type Culture Collection (3 mentions) Penicillin, by Thermo Fisher Scientific (2 mentions) Streptomycin, by Thermo Fisher Scientific (2 mentions) Lipofectamine rnaimax reagent, by Thermo Fisher Scientific (1 mentions) Pcmv6kn, by OriGene (1 mentions) Turbofectin transfection reagent, by OriGene (1 mentions) Keratinocyte sfm, by Thermo Fisher Scientific (1 mentions) Eclipse te20000 s, by Nikon (1 mentions) Hk 2 cells, by Thermo Fisher Scientific (1 mentions) Hk 2 cell, by American Type Culture Collection (1 mentions) Regm bulletkit, by Cambrex (1 mentions) Atcc crl 1573, by American Type Culture Collection (1 mentions) Cycloheximide (chx), by Fujifilm (1 mentions) Tunicamycin t7765, by Merck Group (1 mentions) Cpi 455, by Cayman Chemical (1 mentions) Dmem high glucose, by Nacalai Tesque (1 mentions) Actinomycin d, by Fujifilm (1 mentions)

7 protocols using pcs 400 030

1

NRF2 Knockout in Renal Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
RPTECs were grown and maintained in renal epithelial cell basal medium (PCS-400-030; ATCC) using the renal epithelial cell growth kit (PCS-400-040; ATCC) according to the manufacturer’s instructions. RPTECs for NRF2 KO were transfected with 0, 25, 50, and 100nM NRF2 siRNA (Silence® Select siRNA, s9491, Invitrogen, Carlsbad, CA, USA) using Lipofectamine RNAiMAX reagent (Thermo Fisher Scientific, Waltham, MA, USA) for 24 h, and NRF2 expression was measured after siRNA treatment (Figure S3).
Jo M.J., Kim J.E., Bae S.Y., Cho E., Ahn S.Y., Kwon Y.J, & Ko G.J. (2023). Impaired NRF2 Inhibits Recovery from Ischemic Reperfusion Injury in the Aging Kidney. Antioxidants, 12(7), 1440.
+ Open protocol
+ Expand
2

Isolating and Analyzing Proximal Tubular Cells from Kidney-Specific GLUT2 Knockout Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
We isolated primary proximal tubular epithelial cells from mice with Glut2 knocked out specifically in the kidneys (Ks-Glut2 KO mice) and their littermate controls using a published protocol [33 (link)]. We cultured the cells in collagen-coated six-well plates (106 cells/well; A1142801; Thermo Fisher Scientific, USA) in renal epithelial cell basal medium (PCS-400-030; ATCC, USA) supplemented with the renal epithelial cell growth kit (PCS-400-040; ATCC). Two days after initiating the culture of these cells, we used them for either gene expression measurements or glucose transport assay. For gene expression studies, we transfected the cells with Hnf1α-expressing plasmid (MC202766; OriGene Technologies, USA), control plasmid (PCMV6KN; OriGene Technologies) or Glut2-expressing plasmid (MG208388; OriGene Technologies) using TurboFectin transfection reagent (TF81001; OriGene Technologies) according to the manufacturer’s protocol. Three days after the transfection, we extracted the RNA and used RT-qPCR to measure the mRNA levels of Glut2, Sglt2 and Hnf1α.
de Souza Cordeiro L.M., Bainbridge L., Devisetty N., McDougal D.H., Peters D.J, & Chhabra K.H. (2022). Loss of function of renal Glut2 reverses hyperglycaemia and normalises body weight in mouse models of diabetes and obesity. Diabetologia, 65(6), 1032-1047.
+ Open protocol
+ Expand
3

Epithelial-Mesenchymal Transition in Human Proximal Tubular Epithelial Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human PTECs (ATCC PCS-400-010) were cultured in renal epithelial cell basal medium (ATCC PCS400030™) plus 0.5% fetal bovine serum (FBS), according to the manufacturer’s suggestion. HK-2 cells were purchased from the American Type Culture Collection (Manassas, VA, USA). HK-2 cells were cultured in keratinocyte-SFM (GIBCO) with supplemented 2% FBS. Cells were cultured under normoxia (O2, 21%) and hypoxia (O2, 1%).
The characteristics of EMT in human PTECs were identified by serial observations of their morphological changes using light microscopy (Nikon ECLIPSE TE20000-S, Nikon, Tokyo, Japan).
Kuo M.C., Chang W.A., Wu L.Y., Tsai Y.C, & Hsu Y.L. (2021). Hypoxia-Induced Epithelial-to-Mesenchymal Transition in Proximal Tubular Epithelial Cells through miR-545-3p–TNFSF10. Biomolecules, 11(7), 1032.
+ Open protocol
+ Expand
4

Primary Tubular Epithelial Cell Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary proximal tubular epithelial cells (PTECs) of a DM patient and a normal individual (Lonza Walkersville Inc., Walkersville, MD, USA) were cultured in Clonetics™ REGM™ BulletKit™ (CC-3190). Human PTECs (ATCC PCS-400-010) were cultured in Renal Epithelial Cell Basal Medium (ATCC PCS400030™) plus 0.5% Fetal Bovine Serum (FBS), according to the manufacturer’s suggestion. Cells were treated with normal glucose (NG, 6.2 mM) and HG (30 mM) for the indicated times. Human embryonic kidney (HEK) 293 cells (ATCC® CRL-1573™) were purchased from the American Type Culture Collection (Manassas, VA, USA) and were cultured in DMEM containing 10% FBS.
Tsai Y.C., Kuo P.L., Kuo M.C., Hung W.W., Wu L.Y., Chang W.A., Wu P.H., Lee S.C., Chen H.C, & Hsu Y.L. (2018). The Interaction of miR-378i-Skp2 Regulates Cell Senescence in Diabetic Nephropathy. Journal of Clinical Medicine, 7(12), 468.
+ Open protocol
+ Expand
5

Culturing cell lines for drug screening

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
All cell lines except for HepG2 except and the gene-edited MEFs43 (link) were obtained from ATCC (Manassas, VA, USA). HepG2 cell identity was estimated to be 100% by the Centre for Translational Research and Diagnostics of the National University of Singapore. All cell lines were cultured in high glucose DMEM (Nacalai, Kyoto, Japan) supplemented with 10% FBS, 2 mM glutamine, 100 U/ml penicillin and 100 μg/ml streptomycin (Thermo Fisher Scientific, Waltham, MA, USA). Primary renal proximal tubule epithelial cells (PRETEC) (ATCC, PCS-400-010) were cultured in the recommended media (ATCC, PCS-400-030 and PCS-400-040) and used within tenfold expansion in cell number. GSK-J4 (12073) and CPI-455 (22127) were obtained from Cayman Chemical (Ann Arbor, MI, USA). Tunicamycin (T-7765) was from Sigma-Aldrich (St Louis, MO, USA). Cycloheximide and actinomycin D were from FUJIFILM Wako (Osaka, Japan). The final DMSO concentration was adjusted to 0.2% in all experiments with inhibitor treatment.
Kitajima S., Sun W., Lee K.L., Ho J.C., Oyadomari S., Okamoto T., Masai H., Poellinger L, & Kato H. (2021). A KDM6 inhibitor potently induces ATF4 and its target gene expression through HRI activation and by UTX inhibition. Scientific Reports, 11, 4538.
+ Open protocol
+ Expand
6

Primary Human Renal Proximal Tubule Epithelial Cell Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary human renal proximal tubule epithelial cells were purchased from ATCC (PCS-400-010) and cultured immediately following arrival. No additional authentications or tests were performed on any cell lines. Complete growth medium was prepared as per ATCC recommendations using renal epithelial basal medium (ATCC, PCS-400-030), renal epithelial growth kit (ATCC, PCS-400-040) and Pen-Strep.
Maji A., Soutar C.P., Zhang J., Lewandowska A., Uno B.E., Yan S., Shelke Y., Murhade G., Nimerovsky E., Borcik C.G., Arango A.S., Lange J.D., Marin-Toledo J.P., Lyu Y., Bailey K.L., Roady P.J., Holler J.T., Khandelwal A., SantaMaria A.M., Sanchez H., Juvvadi P.R., Johns G., Hageman M.J., Krise J., Gebremariam T., Youssef E.G., Bartizal K., Marr K.A., Steinbach W.J., Ibrahim A.S., Patterson T.F., Wiederhold N.P., Andes D.R., Pogorelov T.V., Schwieters C.D., Fan T.M., Rienstra C.M, & Burke M.D. (2023). Tuning sterol extraction kinetics yields a renal-sparing polyene antifungal. Nature, 623(7989), 1079-1085.
+ Open protocol
+ Expand
7

Culture of Human Renal Proximal Tubular Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
human renal proximal tubular epithelial cells (hRPTECs) were purchased from the American Type Culture Collection (#PCS-400-010™, Manassas, VA, USA). These cells were grown in 75 cm2 flasks in Renal Epithelial Cell Basal Medium (PCS-400-030™, ATCC®) supplemented with Renal Epithelial Cell Growth Kit (PCS-400-040™, ATCC®).
Kim B.W., Kim H.J., Kim S.H., Baik H.J., Kang M.S., Kim D.H., Markowitz S.D., Kang S.W, & Bae K.B. (2021). 15-Hydroxyprostaglandin dehydrogenase inhibitor prevents contrast-induced acute kidney injury. Renal Failure, 43(1), 168-179.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!