Hml gal4

Manufactured by Bloomington Drosophila Stock Center

The Hml-GAL4 is a genetic tool used in Drosophila research. It is a promoter construct that drives the expression of the yeast transcriptional activator GAL4 protein in specific cell types, allowing for targeted gene expression and manipulation in fruit flies.

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2 protocols using hml gal4

Genetic Fly Lines for Developmental Analysis

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Flies were raised on standard media. Crosses were raised at 25°C unless otherwise noted. 10xstat92E-GFP (BL#26197) [22 (link)], UAS-eGFP (BL#5430), UAS-eGFP (BL#5431), hsflp¹²²;;Ubi-RFP,FRT79 (made from BL#34498), y¹v¹hop^Tum/FM7c (BL#8492; referred to as hop^Tum-l), act5c-GAL4/CyO (BL#4414), UAS-MYR-RFP/CyO (BL#7118), hml-GAL4 (BL#30139) and stat⁰⁶³⁴⁶ (BL# 11681) were obtained from Bloomington Drosophila Stock Center, Bloomington, IN. UAS-hipk^RNAi (VDRC ID#108254, [23 (link)]) was obtained from Vienna Drosophila Resource Center, Vienna, Austria. Also used were dpp-GAL4/TM6B [24 (link)], os,y (a gift from Norbert Perrimon), UAS-Stat92E-GFP/Cyo and UAS-Stat92E-MYC/Cyo,wg-lacZ (a gift from James Castelli-Gair Hombria, [25 (link),26 (link)], PD-lacZ (a gift from Henry Sun; referred to as upd¹-lacZ hereon after, Tsai and Sun, 2004 [27 (link)]), ywhsflp,tub-GAL4,UAS-GFP,6X MYC-NLS; UAS-y+;tub-GAL80,FRT2A/TM6B (a gift from Gary Struhl), ywhsflp¹²²;sp/Cyo;TM2/TM6B, UAS-HA-hipk^1M, UAS-HA-hipk^3M, hipk⁴,FRT79/TM6B [28 (link)], UAS-HA-hipk^WT-attP40, UAS-MYR-HA-hipk-attP40, UAS-NLS-HA-hipk-attP40 (made in this study). act5c-GAL4/CyO and UAS-MYR-RFP/CyO were recombined to generate act5c-GAL4, UAS-MYR-RFP/CyO. hipk⁴, FRT79/TM6B and 10xstat92E-GFP/TM6B were recombined to generate hipk⁴, FRT79,10xstat92E-GFP/TM6B.

Tettweiler G., Blaquiere J.A., Wray N.B, & Verheyen E.M. (2019). Hipk is required for JAK/STAT activity during development and tumorigenesis. PLoS ONE, 14(12), e0226856.

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Drosophila Genetics Techniques Protocol

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Stocks were raised on standard cornmeal-agar medium. Stag 17 embryos, 3rd instar larvae, 3- to 6-days old adults were used in this study. w1118 flies were used as a standard wild-type strain. Hml-Gal4 (stock ID 30141), Hml > GFP (stock ID 30142), en-Gal4 (stock ID 83350), sev-Gal4 (stock ID 5793), UAS-Tak1 (stock ID 58810), Tak1² (stock ID 26272), Atg1 RNAi (stock ID 26731), Atg101 RNAi (stock ID 34360) and ban-lacZ (stock ID 10154) lines were obtained from Bloomington Drosophila Stock Center (BDSC). Tak1 RNAi (stock ID 101357), Tao-1 RNAi (stock ID 17432), Hpo RNAi (stock ID 104169), Wts RNAi (stock ID 106174), Atg13 RNAi (stock ID 27955) and Yki RNAi (stock ID 40497) lines were collected from Vienna Drosophila Resource Center (VDRC). Atg9 RNAi (stock ID THU2895) was collected from TsingHua Fly Center. UAS-Yki line has been reported previously^{85 (link)}.

Yang Y., Zhou H., Huang X., Wu C., Zheng K., Deng J., Zheng Y., Wang J., Chi X., Ma X., Pan H., Shen R., Pan D, & Liu B. (2024). Innate immune and proinflammatory signals activate the Hippo pathway via a Tak1-STRIPAK-Tao axis. Nature Communications, 15, 145.

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