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Pegfp n1 human cofilin wt

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PEGFP-N1 human cofilin WT is a plasmid that encodes the wild-type human cofilin protein fused to green fluorescent protein (GFP). Cofilin is an actin-binding protein involved in the regulation of actin dynamics within cells.

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Lab products found in correlation

Pmrfp n1 human cofilin wt, by Addgene (2 mentions) Lck gfp, by Addgene (1 mentions) Megfp lifeact 7, by Addgene (1 mentions) Egfp actin, by Takara Bio (1 mentions) Ptdtomato n1, by Takara Bio (1 mentions) Pmcherry c1, by Takara Bio (1 mentions) Plenti pgk puro dest, by Addgene (1 mentions) Eb1 gfp, by Addgene (1 mentions) Hdac6 flag, by Addgene (1 mentions) Ecfp ssh1δc, by Addgene (1 mentions) Poptn egfp, by Addgene (1 mentions) Pmxs puro gfp p62, by Addgene (1 mentions)

3 protocols using pegfp n1 human cofilin wt

1

Plasmid Verification and Utilization

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All plasmids were acquired from Addgene and verified by sequencing using the associated primers on the Addgene website. The following plasmids were used: LCK-GFP (Addgene 61099), LCK-mScarlet-1 (Addgene 98821), pEGFP-N1 human cofilin WT (Addgene 50859), tdTomato-Lifeact-7 (Addgene 54528), mEGFP-Lifeact-7 (Addgene 54610), and pmRFP-N1 human cofilin WT (Addgene 50856).
Hylton R.K., Heebner J.E., Grillo M.A, & Swulius M.T. (2022). Cofilactin filaments regulate filopodial structure and dynamics in neuronal growth cones. Nature Communications, 13, 2439.
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2

Mammalian Expression Plasmid Library

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The following mammalian expression plasmids have been described: actin-eGFP (Clontech), EB1-GFP (Addgene plasmid 17234), CTTN-FLAG (gift from Dr Yingtao Zhang, University of Florida), pmRFP-N1 human cofilin WT (Addgene plasmid 50856), pmRFP-N1 human cofilin SA (Addgene plasmid 50857), pmRFP-N1 human cofilin WT (Addgene plasmid 50855), pEGFP-N1 human cofilin WT (Addgene plasmid 50859), pEGFP-N1 human cofilin SA (Addgene plasmid 50854), pEGFP-N1 human cofilin SE (Addgene plasmid 50861), HDAC6 Flag (Addgene plasmid 13823), pLenti PGK Puro DEST (Addgene, plasmid w529-2).
Flag-Cofilin was generated by cloning cofilin coding sequence from pLenti PGK Puro DEST (Addgene, plasmid w529-2) into pENTR4-Flag gateway entry vector (Clontech plasmid 17423) and subsequently to pcDNA™3.2-DEST destination vector (Clontech plasmid). Actin-mCherry was generated by cloning actin coding sequence from actin-eGFP into pmCherry-C1 (Clontech). Cortactin-tdTomato (CTNN-tdTom) was generated by cloning CTTN coding sequence from CTTN-eGFP into ptdTomato-N1 (Clontech). Cofilin K33, 44, 96, 144R (cofilin KR) (one construct with 4 point mutations) and cofilin K33, 44, 96, 144Q (cofilin KQ) (one construct with 4 point mutations) were generated from Flag-Cofilin using site directed or multi-site directed mutagenesis kits (QuickChange II).
Zalli D., Neff L., Nagano K., Shin N.Y., Witke W., Gori F, & Baron R. (2016). The Actin-Binding Protein Cofilin and Its Interaction With Cortactin Are Required for Podosome Patterning in Osteoclasts and Bone Resorption In Vivo and In Vitro. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 31(9), 1701-1712.
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3

Plasmid Constructs for Autophagy and Cytoskeleton Research

Cited 3 times
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pMXs-puro GFP-p62 (Addgene, 38277) (Itakura and Mizushima, 2011 (link)), pMXs-puro GFP-p62ΔC (addgene, 38282) (Itakura and Mizushima, 2011 (link)), ECFP-SSH1ΔC (Kurita et al., 2008 (link)), pEGFP-N1 human cofilin WT (addgene, 50859) (Garvalov et al., 2007 (link)), pEGFP-N1 human cofilin S3A (addgene, 50860) (Garvalov et al., 2007 (link)), pEGFP-N1 human cofilin S3E (addgene, 50861) (Garvalov et al., 2007 (link)), pOPTN-EGFP (addgene, 27052) (Park et al., 2006 (link)), pOPTN E478G-EGFP (addgene, 68848) (Turturro et al., 2014 (link)) were obtained from corresponding sources. Plasmids p3xFlag-SSH1 and p3xFlag-SSH1ΔN, and GFP-p62 403E were generated in the Kang lab as previously documented (Fang et al., 2021 (link)).
Cazzaro S., Fang C., Khan H., Witas R., Kee T.R., Woo J.A, & Kang D.E. (2022). Slingshot homolog-1 mediates the secretion of small extracellular vesicles containing misfolded proteins by regulating autophagy cargo receptors and actin dynamics. Frontiers in Aging Neuroscience, 14, 933979.
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