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Prime script cdna synthesis kits

Manufactured by Thermo Fisher Scientific
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Sourced in United States

The Prime-Script cDNA Synthesis Kits are used for the reverse transcription of RNA into complementary DNA (cDNA). The kits contain necessary reagents, including a reverse transcriptase enzyme, to facilitate the conversion of RNA into single-stranded cDNA molecules.

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Lab products found in correlation

Sybr green pcr kit, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

CDNA synthesis kit (1264 citations) CDNA kits (3 citations)

2 protocols using prime script cdna synthesis kits

1

RNA Extraction and cDNA Synthesis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was extracted using RNA-Quick Purification Kit (ES Science, Guangzhou, China) and cDNA synthesis using the Prime-Script cDNA synthesis kits (Invitrogen, California, USA) according to the manufacturer’s instructions. The reverse-transcribed cDNA products were used for qPCR analysis using SYBR Green PCR kit (Invitrogen, California, USA). Supplementary Table 2 includes detailed information about the sequence of the used primers.
Shi Y., Niu Y., Yuan Y., Li K., Zhong C., Qiu Z., Li K., Lin Z., Yang Z., Zuo D., Qiu J., He W., Wang C., Liao Y., Wang G., Yuan Y, & Li B. (2023). PRMT3-mediated arginine methylation of IGF2BP1 promotes oxaliplatin resistance in liver cancer. Nature Communications, 14, 1932.
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2

Total RNA Extraction and qPCR Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was extracted using RNA-Quick Purification Kit (ES Science, Guangzhou, China) and cDNA was synthesized using the Prime-Script cDNA synthesis kits (Invitrogen, CA, USA) according to the manufacturer’s instructions. The reverse-transcribed cDNA products were used for qPCR analysis using the SYBR Green PCR kit (Invitrogen, California, USA).
Ma M., Duan Y., Peng C., Wu Y., Zhang X., Chang B., Wang F., Yang H., Zheng R., Cheng H., Cheng Y., He Y., Huang J., Lei J., Ma H., Li L., Wang J., Huang X., Tang F., Liu J., Li J., Ying R., Wang P., Sha W., Gao Y., Wang L, & Ge B. (2024). Mycobacterium tuberculosis inhibits METTL14-mediated m6A methylation of Nox2 mRNA and suppresses anti-TB immunity. Cell Discovery, 10, 36.
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