Anti gapdh antibody 10r 2932

Manufactured by Biosynth

Sourced in United States

The Anti-Gapdh antibody (10R-2932) is a laboratory reagent used for the detection and quantification of the Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) protein. GAPDH is a commonly used housekeeping gene and is involved in the glycolytic pathway. This antibody can be used in various immunoassay techniques, such as Western blotting, to identify and measure GAPDH levels in biological samples.

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Close spelling variants of this product

GAPDH (73 citations) Anti-GAPDH (20 citations) GAPDH antibody (2 citations)

2 protocols using anti gapdh antibody 10r 2932

Western Blot Analysis of Signaling Proteins

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Anti-Smad2 (#5339), p-Smad2 (Ser465/467 #3108), p-Akt (Thr308, #2965), p-Akt (Ser473, #4060), NFkB (#3037), p-Erk (Ser259 #9911), p-p38 (#4511), p-Jnk (#4668), p-Stat3 (#9145), p-Stat1 (#9171), Tgfbr2 (#3713), p-IRS1 (Tyr895, 3070), p-Smad1/5(#9511), p-Pten (Ser380/Thr382/383, #9549), Pten (D4.3 #9188), and Act-b (13E5, #4970) antibodies were purchased from Cell Signaling Technology. Anti-p-Akt (T308) antibody (# 658320) was purchased from R&D Systems. Anti-Mycn (B8.4.B, #sc-53993) antibody was purchased from Santa Cruz Biotechnology. Anti-pCKIIβ (S209) antibody (STJ90892) was purchased from St Joh’s laboratory. Anti-Gapdh antibody (10R-2932) was purchased from Fitzgerald Industries International. Western blot analysis was performed according to the standard procedure. Uncropped blots are included in Supplemetary Figs. 9–12.

Mirzamohammadi F., Kozlova A., Papaioannou G., Paltrinieri E., Ayturk U.M, & Kobayashi T. (2018). Distinct molecular pathways mediate Mycn and Myc-regulated miR-17-92 microRNA action in Feingold syndrome mouse models. Nature Communications, 9, 1352.

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Western Blot Analysis of Protein Targets

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Anti-MEF2C (#5030), anti-HDAC5 (#2082), anti-Histone 2A (#2578), and anti-Phospho-HDAC4 (Ser246)/HDAC5 (Ser259)/ HDAC7 (Ser155)(#3443) antibodies were purchased from Cell Signaling Technology, Denvers, MA, USA. Anti-MEF2D (BDB610774) was from BD Biosciences, San Jose, CA, USA, Anti-HDAC4 antibody (ab12172) was purchased from Abcam. AntiRalA antibody was from GeneTex, Irvine, CA, USA. Anti-Sox9 antibody (bs-4177R) was from Bioss, Woburn, MA, USA. Anti-Actin antibody (I–19) was purchased from Santa Cruz, Dallas, Texas, USA Biotechnology. Anti-GAPDH antibody (10R-2932) was purchased from Fitzgerald Industries International, Acton, MA, USA. Western blot analysis was performed according to the standard procedure.

Papaioannou G., Mirzamohammadi F., Lisse T.S., Nishimori S., Wein M.N, & Kobayashi T. (2015). MicroRNA-140 Provides Robustness to the Regulation of Hypertrophic Chondrocyte Differentiation by the PTHrP-HDAC4 Pathway. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 30(6), 1044-1052.

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