E. coli BL21 cells and pET28a vector were purchased from Novagen (Darmstadt, Germany). pBT, pTRG vectors and E. coli XR host strains were purchased from Stratagene (La Jolla, CA, USA) (Additional file 4 : Table S1). Restriction enzymes, T4 ligase, Modification enzymes, Pyrobest DNA polymerase, dNTPs and all antibiotics were obtained from TaKaRa Biotech (Shiga, Japan). The reagents for one-hybrid assay were purchased from Stratagene. Polymerase Chain Reaction (PCR) primers were synthesized by Invitrogen (Carlsbad, CA, USA) (Additional file 4 : Table S2).
Modification enzymes
Manufactured by Takara Bio
Sourced in Japan
Modification enzymes are a class of enzymes used to manipulate and alter DNA or RNA molecules. These enzymes can perform various functions, such as cutting, joining, or modifying nucleic acid sequences. They are essential tools in molecular biology, genetic engineering, and biotechnology research.
Automatically generated - may contain errors
Lab products found in correlation
T4 ligase, by Takara Bio (3 mentions)
Restriction enzyme, by Takara Bio (3 mentions)
Dntps, by Takara Bio (3 mentions)
Dna polymerase, by Takara Bio (2 mentions)
Ni nta ni2 nitrilotriacetate agarose, by Qiagen (2 mentions)
7h9 and 7h10 broths, by BD (2 mentions)
Pyrobest dna polymerase, by Takara Bio (1 mentions)
3 protocols using modification enzymes
1
Bacterial Expression System Setup
2
Protein Expression Using E. coli BL21
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E. coli BL21 (λDE3) cells and pET28a were purchased from Novagen (Darmstadt, Germany) and were used to express proteins. Restriction enzymes, T4 ligase, modification enzymes, DNA polymerase, dNTPs, and all antibiotics were obtained from TaKaRa Biotech (Shiga, Japan). PCR primers were synthesized by Invitrogen (Carlsbad, USA). Ni-NTA (Ni2+-nitrilotriacetate) agarose was purchased from Qiagen (Hilden, Germany). 7H9 and 7H10 broths were purchased from Becton, Dickinson Company (New Jersey, USA). Antibodies were obtained from the Wuhan laboratory animal center of CAS (Wuhan, China).
3
Recombinant Protein Expression in E. coli
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Escherichia coli (E. coli) BL21 (λ DE3) and pET28a were purchased from Novagen (Darmstadt, Germany) and used to express proteins. Restriction enzymes, T4 ligase, modification enzymes, DNA polymerase, dNTPs were obtained from TaKaRa Biotech (Shiga, Japan). PCR primers were synthesized by Invitrogen (Carlsbad, USA). Ni-NTA (Ni2+-nitrilotriacetate) agarose was purchased from Qiagen (Hilden, Germany). 7H9 and 7H10 broths were purchased from Becton, Dickinson Company (New Jersey, USA). Cordycepin (3’-deoxyadenosine, from Cordyceps militaris, C2689) was purchased from Tokyo chemical industry CO., LTD. (Tokyo, Japan).
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