Sup b15

The human Ph⁺ ALL cell line SUP-B15 and human embryonic kidney cell line 293T were purchased from The American Type Culture Collection. The SUP-B15 cell line has been authenticated by short tandem repeat DNA profiling analysis by Genetic Testing Biotechnology Corporation (Suzhou). SUP-B15 cells were cultured in Iscove's modified Dulbecco's medium (IMDM; Sigma-Aldrich; Merck KGaA) with 20% FBS (Gibco; Thermo Fisher Scientific, Inc.), and 293T cells were cultured in DMEM (Sigma-Aldrich; Merck KGaA) with 10% FBS. All cells were maintained in a humidified incubator at 37°C in an atmosphere of 5% CO₂.

The spermatogonia
GC-1 cell line (ATCC CRL-2053)^{55 (link)} derived
from BALB/c mouse testes and the human B-lymphoblastic leukemia cell
line SUP-B15 (ATCC CRL-1929)^{56 (link)} were obtained
from the American Type Cell Culture Collection (ATCC). The GC-1 cell
line was cultured in Dulbecco’s modified Eagle’s medium
(DMEM; Sigma-Aldrich), while the SUP-B15 cell line was grown in RPMI-1640
complete medium (Sigma-Aldrich) supplemented with 10% fetal bovine
serum (Gibco, Dublin, Ireland) and 1% penicillin–streptomycin
(Sigma-Aldrich). Cells were incubated in 5% CO₂ at 37 °C.