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2 protocols using kh2po2

1

Vascular Reactivity Pharmacological Agents

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Phenylephrine, acetylcholine chloride, sodium nitrite, sodium nitroprusside, L-NAME and Tween-20 were purchased from Sigma Chemicals (St Louis, MO, USA). NaCl was purchased from Calbiochem® Merck (Darmstadt, Germany). MgSO4, KCl, KH2PO2, glucose and CaCl2 were purchased from BDH Laboratory Supplies (Poole, UK). Bovine serum albumin (BSA) was purchased from Santa Cruz (Dallas, Texas, USA). All compounds were dissolved in deionized water. The concentrations stated are expressed as final molar concentrations in the buffer. At the concentrations used, the vehicles had no significant effect on vascular responses (data not shown).
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2

Preparation of Pharmacological Reagents

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Phenylephrine, acetylcholine chloride, sodium nitrite, sodium nitroprusside, and Tween-20 were purchased from Sigma Chemicals (St Louis, MO, USA). Angiotensin II was purchased from Sigma Chemicals (USA). EGCG was purchased from Cayman. NaCl was purchased from Calbiochem® Merck (Darmstadt, Germany). MgSO4, KCl, KH2PO2, glucose and CaCl2 were purchased from BDH Laboratory Supplies (Poole, UK). Bovine serum albumin (BSA) was purchased from Santa Cruz (Dallas, Texas, USA). All compounds were dissolved in deionized water. The concentrations stated are expressed as final molar concentrations in the buffer.
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