Circulating levels of PCKS9 were measured on EDTA-plasma samples according to the manufacturer's directions with the PCSK9 Quantikine ELISA Kit (SPC900) from R&D Systems (Minneapolis, MN). The mean minimum detectable dose was 0.096 ng/mL [30 (link)]. The maximum intra- and interassay coefficients of variation were 6.5% and 5.9%, respectively.
Pcsk9 quantikine elisa kit
Manufactured by R&D Systems
The PCSK9 Quantikine ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed to measure human PCSK9 levels in cell culture supernates, serum, and plasma. The kit utilizes a monoclonal antibody specific for PCSK9 that has been pre-coated onto a microplate.
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4 protocols using pcsk9 quantikine elisa kit
1
PCSK9 Quantification in EDTA-Plasma
2
ELISA Quantification of PCSK9 and A1AT
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Proteins were determined in plasma using Mouse Proprotein Convertase 9/PCSK9 Quantikine ELISA Kit (MPC900, R&D Systems) and Mouse A1AT ELISA Kit (E-90A1T, Immunology Consultants Laboratory) according to the manufacturer’s instructions detecting all 5 paralogues of Serpina1.
3
Inhibition of PCSK9 Production
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The compounds PF-06446846 (PF846) and PF-06378503 (PF8503) were synthesized as described in [9 (link)] and provided by Pfizer. To determine the IC50 value for inhibition of PCSK9 production, 100 μL of PF846 and PF8503 dilutions in DMSO (0.5% final concentration in media) were added to an overnight culture of 3000 Huh7 cells/mL. PCSK9 production was estimated after overnight incubation by the determination of PSCK9 concentration using a solid phase sandwich ELISA (PCSK9 Quantikine ELISA Kit, RD systems).
4
PCSK9 Quantification in Mouse Serum
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PCSK9 in mouse serum was quantified with a Mouse Proprotein Convertase 9/PCSK9 Quantikine ELISA Kit (MPC900, R&D Systems) according to the manufacturer’s instructions.
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