Crl 2267

Five NB cell lines were purchased from ATCC (New York, NY). CRL-2267, CCL-127, CRL-2271 (of male origin) CRL-2266, and CRL- 2149 (of female origin) were cultured in Eagle's Minimum Essential Media (EMEM) (ATCC, Manassas, VA) supplemented with 10% heat inactivated Fetal Bovine Serum (FBS), and l-Glutamine-penicillin-streptomycin solution (designated complete media) (Sigma, St. Louis, MO) at 37 °C, 5% CO₂. SMS-KCNR (of male origin) was purchased from Children's Oncology Group (Texas Tech University) and was cultured in RPMI-1640 (Sigma). Two terminally differentiated olfactory neuroblastoma cell lines, T-268 and JFEN, were a kind gift from Timothy J. Triche (Department of Pathology, Children's Hospital of Los Angeles, Los Angeles, CA 90027; 21). The stock cell cultures were grown in 25 mL or 75 mL flasks (Thermo-Scientific, Nunc, Rochester, NY). ZIKV strains PRVABC59 and MR 766 were provided by the CDC (kind gifts from Brandy Russel, Fort Collins, CO) and flavivirus envelop antibody (FE1), which cross-reacts with ZIKV, was purchased from Invitrogen (Cat. # MA1-71258). Two NB cell lines CRL-2266 and CRL-2267 were exposed to retinoic acid (RA) at 1 μM final concentration for 48 h (Breitman et al., 1980 (link)). Both cell lines exhibited partial differentiation as measured by morphologically and by immunostaining with anti-tubulin antibody.