Surveyor plus hplc system

Manufactured by Thermo Fisher Scientific

Sourced in Singapore

The Surveyor Plus HPLC System is a high-performance liquid chromatography (HPLC) instrument designed for analytical applications. It provides accurate and reliable separation and quantification of complex mixtures. The system features precise solvent delivery, automated sample handling, and advanced data processing capabilities.

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Lab products found in correlation

Kinetex biphenyl lc column, by Phenomenex (2 mentions) C18 reverse phase column, by Agilent Technologies (1 mentions) Tsq quantum ultra triple quadrupole mass spectrometer, by Thermo Fisher Scientific (1 mentions) Acquity qda, by Waters Corporation (1 mentions) Tsq quantum access, by Thermo Fisher Scientific (1 mentions)

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HPLC system (141 citations) Surveyor HPLC system (64 citations) Surveyor HPLC (20 citations) Surveyor MS Pump Plus HPLC system (4 citations) Surveyor Plus HPLC (3 citations) PLUSTM (3 citations)

6 protocols using surveyor plus hplc system

Quantification of Riboflavin in Intestinal Samples

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Riboflavin was quantified using HPLC as described previously (50 (link)). Ileum (0.1 g) and colonic contents were weighed and vortexed for 5 min in 1 ml PBS buffer. The samples were centrifuged at 13,000 rpm for 10 min, and the supernatants were collected. The supernatants were then filtered through a 0.22-μm filter membrane. The filtered supernatants were transferred to an HPLC vial and analyzed by HPLC. For the riboflavin standard curve, 0–100 μM riboflavin was prepared in PBS and analyzed by HPLC. HPLC was performed using a reverse-phase C18 column (Agilent, 4.6×250 mm, 5 μm) at room temperature on a Surveyor Plus HPLC System (Thermo Finnigan) by isocratic elution at a flow rate of 1 mL/min using a mobile phase of 1% acetic acid:methanol (70:30, v/v). The sample injection volume was 20 μL. The detection wavelength was 282 nm. The peak area of the analytes in question was measured and used for quantification.
Detailed experimental protocols and materials and methods are described in SI Appendix, Materials and Methods.

Liu B., Liu Y., Yang B., Wang Q., Liu X., Qin J., Zhao K., Li F., Feng X., Li L., Wu P., Liu M., Zhu S., Feng L, & Wang L. (2022). Escherichia coli O157:H7 senses microbiota-produced riboflavin to increase its virulence in the gut. Proceedings of the National Academy of Sciences of the United States of America, 119(48), e2212436119.

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HPLC-MS/MS Analysis of Microdialysis Samples

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A Thermo Finnigan Surveyor Plus HPLC system was used for analyzing microdialysis samples. Neurochemical separation was achieved with a Phenomenex (Torrance, CA) Kinetex biphenyl LC column (50 × 2.1 mm, 1.7 μm particle size, 100 Å pore size). Mobile phase A was 10 mM ammonium formate and 0.15% (v/v) formic acid in HPLC water. Mobile phase B was acetonitrile. The mobile phase gradient for all of the analytes was: initial, 0% B; 0.1 min, 10% B; 0.12 min, 10% B; 2.3 min, 20% B; 3.7 min, 50% B; 4.0 min, 80% B; 4.5 min, 100% B; 5.0 min 100% B; 6.5 min, 0% B. The flow rate was 200 μL/min, and the sample injection volume was 7 μL. The autosampler and column were maintained at ambient temperature throughout the analysis. A Thermo Finnigan TSQ Quantum Ultra triple quadrupole mass spectrometer operating in positive mode was used for detection. Electrospray ionization (ESI) voltage was 3.5 kV, and heated ESI probe (HESI-I) was set at 300°C. Capillary temperature was 350°C, and sheath gas, aux gas, and ion sweep gas were maintained at 25, 15, and 0 arb, respectively. The intercycle delay was 200 ms. Automated peak integration was performed using Thermo X Calibur Quan Browser version 2.1. All peaks were visually inspected to ensure proper integration. Calibration curves were constructed based on peak area ratio (P_analyte/P_IS) versus concentrations by linear regression.

Campus P., Covelo I.R., Kim Y., Parsegian A., Kuhn B.N., Lopez S.A., Neumaier J.F., Ferguson S.M., Solberg Woods L.C., Sarter M, & Flagel S.B. (2019). The paraventricular thalamus is a critical mediator of top-down control of cue-motivated behavior in rats. eLife, 8, e49041.

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HPLC-MS Analysis of Microdialysis Samples

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Microdialysis samples were analyzed by a Thermo Finnigan (San Jose, CA) Surveyor Plus HPLC system consisting of an Autosampler Plus and MS Pump Plus. Neurochemical separation was achieved with a Phenomenex (Torrance, CA) Kinetex biphenyl LC column (50 × 2.1 mm, 1.7 μm particle size, 100 Å pore size). Mobile phase A was 10 mm ammonium formate with 0.15% (v/v) formic acid in water. Mobile phase B was acetonitrile. The mobile phase gradient for all 16 analytes was as follows: initial, 0% B; 0.1 min, 10% B; 2.3 min, 20% B; 3.7 min, 50% B; 4.0 min, 80% B; 4.5 min, 0% B; 6.5 min, 0% B. The flow rate was 200 μL/min, and sample injection volume was 5 μL. The autosampler and column were maintained at ambient temperature throughout the analysis.

Pitchers K.K., Kane L.F., Kim Y., Robinson T.E, & Sarter M. (2017). ‘Hot’ vs. ‘cold’ behavioural-cognitive styles: motivational-dopaminergic vs. cognitive-cholinergic processing of a Pavlovian cocaine cue in sign- and goal-tracking rats. The European journal of neuroscience, 46(11), 2768-2781.

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Quantification of c-di-GMP via HPLC

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c-di-GMP was quantified using HPLC as described previously [45 (link)]. Overnight cultures were subcultured in LB medium at 37 °C until they reached an OD₆₀₀ of 0.6 for adaptation. Approximately 100 mg of cells were harvested in a pellet by centrifugation. The pellet was washed with PBS and resuspended in H₂O. The suspension was heated at 95 °C for 15 min, followed by sonication. Ethanol was added to the sample to a final concentration of 70%. After centrifugation, the supernatant was pooled, frozen, and subsequently lyophilized overnight. The lyophilized flakes were resuspended in 1 ml of H₂O and filtered through a 0.2 µm pore size filter. HPLC was performed using a 5 μm, 4.6 × 250 mm reverse phase column (Agela Venusil XBP-C18, VX952505-0) at room temperature with detection at 253 nm, on a Surveyor Plus HPLC System (Thermo Finnigan). Each experiment was carried out at least three times.

Liu Y., Li S., Li W., Wang P., Ding P., Li L., Wang J., Yang P., Wang Q., Xu T., Xiong Y, & Yang B. (2019). RstA, a two-component response regulator, plays important roles in multiple virulence-associated processes in enterohemorrhagic Escherichia coli O157:H7. Gut Pathogens, 11, 53.

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Identification of Kefir Metabolites by LC-MS

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The kefir biomass crude extract was dissolved in Acetonitrile and injected to LC-MS to identify molecules that originated from the Kluyveromyces marxianus metabolism. The molecular weight was determined by MS using an LTQ XL Orbitrap with a static nanospray in positive ion mode (Waters Acquity QDA with PDA and QDA detectors) analyzed by Xcalibur and Process software (Thermo Scientific). For LC/MS analyses, a Surveyor Plus HPLC System (Thermo Scientific) was used, equipped with a Luna C18, 5 μm (150 × 4.6 mm) column at a flow rate of 0.5 mL/min, using a mobile phase linear gradient of 0.1% aqueous formic acid (solvent A) and acetonitrile containing 0.1% formic acid (solvent B). Additionally, quantitative determination of tryptophol acetate concentration in the kefir biomass crude extract was implemented. To do so, we diluted the Tryptophol acetate at six concentrations: 0.01, 0.02, 0.05, 0.1, 0.25, and 0.50 mM to generate a calibration curve. We produced a plot of tryptophol acetate peak area vs. concentration and the plot showed a linear relationship.

Malka O., Kalson D., Yaniv K., Shafir R., Rajendran M., Ben-David O., Kushmaro A., Meijler M.M, & Jelinek R. (2021). Cross-kingdom inhibition of bacterial virulence and communication by probiotic yeast metabolites. Microbiome, 9, 70.

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HPLC-MS/MS Analysis of Ursolic Acid Glucoside

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HPLC separation was performed using a Surveyor plus HPLC system (Thermo Fisher, Singapore) equipped with a Hypersil Golden C₁₈ column (100 mm × 2.1 mm, 3 μm particles) at 20°C. The mobile phase consisted of water (component A) and methanol (component B) and the flow rate was 0.2 mL min^–1. The gradient program was: 0–2.0 min, 30% B; 2.0–5.0 min, 30–80% B; 5.0–13.0 min, 80% B; 13.0–15.0 min, 80–90% B; 15.0–25.0 min, 90% B. HESI-MS/MS data were acquired with a triple quadrupole mass spectrometer (TSQ Quantum Access, Thermo Fisher, United States) coupled with an electrospray source in positive ionization mode. The MS parameters were set as follows: vaporizer temperature 375°C, capillary temperature 300°C, sheath gas 40 (arbitrary units), aux gas 5 (arbitrary units) and spray voltage 3.5 kV. For qualitative and semi-quantitative analysis of metabolites, selected reaction monitoring (SRM) mode was used. The contents of ursolic acid 28-O-β-D-glucopyranoside were determined by calculating relative peak areas using the product prepared from IaAU1 enzymatic activity assay as standard.

Ji X., Lin S., Chen Y., Liu J., Yun X., Wang T., Qin J., Luo C., Wang K., Zhao Z., Zhan R, & Xu H. (2020). Identification of α-Amyrin 28-Carboxylase and Glycosyltransferase From Ilex asprella and Production of Ursolic Acid 28-O-β-D-Glucopyranoside in Engineered Yeast. Frontiers in Plant Science, 11, 612.

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