Stericup quick release vacuum

The young and green leaves of S. blepharophylla and S. greggii collected were cleaned by rinsing in distilled water several times. Then, leaves were freeze-dried using a BenchTop Pro with Omnitronics™ freeze dryer (BTP-8ZL00W, SP Scientific, Warminster, PA, USA) and were grinded using an electrical blender. Subsequently, the leaf powder (5 g each) was added to flasks containing 500 mL Milli-Q water (18.2 MΩ cm) and was shaken with an orbital shaker (IKA Basic Variable-Speed Digital Orbital Shaker, model, 115 V) at 200 rpm at 35 °C for 72 h followed by sonication using an ultrasonic bath (Bransonic^® MH-series, CPX-952-217R, Danbury, CT, USA) for 90 min and maceration for 2 h at room temperature. The extract solution was filtered using a Stericup^® Quick Release Vacuum-driven disposable filter integrated with Millipore Express^® Plus 0.22 µm PES System (Sigma Aldrich, St-Louis, MO, USA). The filtrates were kept at 4 °C pending the synthesis of AgNPs.

Green leaves of pecan (Carya illinoinensis (Wangenh). K. Koch) were collected from the Bill and Vara Daniel Farm and Ranch located at Prairie View A&M University (PVAMU) and the sample was identified and the voucher specimen was stored at the Cooperative Agricultural Research Center (CARC) at Prairie View A&M University. The collected leaves were cleaned by rinsing in distilled water several times to remove debris. Subsequently, the leaves were freeze-dried using BenchTop Pro with Omnitronics™ freeze dryer (BTP-8ZL00W, SP Scientific, PA, USA) and grounded manually using mortar and pestle. Next, the fine leave powder (15 g) was added to 400 mL deionized water and shaken with an orbital shaker (IKA Basic Variable-Speed Digital Orbital Shaker, model, 115 V) at 200 rpm at 30°C for 48 hrs. The extract solution was filtered using Stericup^® Quick Release Vacuum driven disposable filter (integrated with Millipore Express^® Plus 0.22 µm PES System (Sigma Aldrich). The filtrate was kept at 4°C pending the synthesis of ZnO NPs.