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Nt1 13

Manufactured by Merck Group

The NT1–13 is a versatile laboratory instrument designed for performing a wide range of analytical and research tasks. It features a compact and durable construction, making it suitable for use in various laboratory settings. The NT1–13 is capable of accurate and precise measurements, but its specific intended use is not provided in this factual description.

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2 protocols using nt1 13

1

Optimizing Metabolic Signaling in Cell Studies

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Phospho-AMPKα (Thr172) (2535), AMPKα (2532), LKB1 (3050), phospho-Akt (Ser-473) (4058) and pan-Akt (4691) antibodies were from Cell Signaling Technology (Danvers, MA). NTR1 antibody (sc-374492) was from Santa Cruz Biotechnology (Dallas, TX). CaMKK2 (ab168818), NTR3 (ab16640), and F4/80 (ab100790) antibodies were from Abcam (Cambridge, MA). FLAG (F1804) and β-actin (A5316) antibodies were from Sigma-Aldrich (St. Louis, MO). AICAR was from Cayman (Ann Arbor, MI). Oleate sodium, NT1–13, glucose and human insulin were from Sigma. Deuterated oleic acid (CLM-460-PK) was obtained from Cambridge Isotope Laboratories (Tewksbury, MA). SR 48692 was from Tocris (Minneapolis, MN). Lipofectamine® RNAiMAX and LTX Reagent with PLUS transfection reagents and Trizol were from Life Technologies (Grand Island, NY). pSG5-FLAG-CaMKK2 rat FL was a gift from Anthony Means30 (link) (Addgene plasmid #32449, Cambridge, MA). Primers for RT-PCR were from Integrated DNA Technologies (Coralville, IA).
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2

Optimizing Metabolic Signaling in Cell Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols
Phospho-AMPKα (Thr172) (2535), AMPKα (2532), LKB1 (3050), phospho-Akt (Ser-473) (4058) and pan-Akt (4691) antibodies were from Cell Signaling Technology (Danvers, MA). NTR1 antibody (sc-374492) was from Santa Cruz Biotechnology (Dallas, TX). CaMKK2 (ab168818), NTR3 (ab16640), and F4/80 (ab100790) antibodies were from Abcam (Cambridge, MA). FLAG (F1804) and β-actin (A5316) antibodies were from Sigma-Aldrich (St. Louis, MO). AICAR was from Cayman (Ann Arbor, MI). Oleate sodium, NT1–13, glucose and human insulin were from Sigma. Deuterated oleic acid (CLM-460-PK) was obtained from Cambridge Isotope Laboratories (Tewksbury, MA). SR 48692 was from Tocris (Minneapolis, MN). Lipofectamine® RNAiMAX and LTX Reagent with PLUS transfection reagents and Trizol were from Life Technologies (Grand Island, NY). pSG5-FLAG-CaMKK2 rat FL was a gift from Anthony Means30 (link) (Addgene plasmid #32449, Cambridge, MA). Primers for RT-PCR were from Integrated DNA Technologies (Coralville, IA).
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