Nad and β nicotinamide mononucleotide

Radioactive [α-³²P] ATP was produced in the Laboratory of Biotechnology at ICBFM (Siberian Branch of Russian Academy of Sciences [SB RAS], Novosibirsk, Russia). Oligodeoxynucleotides were synthesized by Biosset (Russia) and the Laboratory of Biomedical Chemistry at ICBFM (SB RAS, Novosibirsk, Russia). NAD⁺ and β-nicotinamide mononucleotide were purchased from Sigma-Aldrich (United States, catalog # 481911 and N3501, respectively), whereas reagents for buffer and electrophoresis components from Sigma-Aldrich, United States (Tris, catalog #T6791; BSA, catalog # A9418; EDTA, catalog #E5134; HEPES, catalog #H3375), PanReacAppliChem, Germany (acrylamide/bis-acrylamide, catalog # A1089/A3636; Urea catalog # A1049), Molecular Group (DTT, catalog # 19733320), Merk (NaCl, catalog # 106404).

Recombinant histidine-tagged analogs of YB-1 protein and its nuclear form YB-1(1-219) were produced by expression in Escherichia coli BL21(DE3) and purified as described previously [76 (link)]. Recombinant PARP1 was purified as described earlier with minor modifications [77 (link)]. The plasmid pET-3-1-YB-1 containing the human YB-1 cDNA was generous gift from Lev P. Ovchinnikov and Dmitry Kretov (Institute of Protein Research RAS, Moscow, Russia). The plasmid DNA containing the human PARP1 cDNA was a kind gift of Dr. M. Satoh (Université Laval, Québec, Canada).
Yeast nicotinamide mononucleotide adenylyltransferase (NMAT) and phage T4 polynucleotide kinase were kindly provided by Dr. Stanislav I. Shram (IMG RAS, Moscow, Russia) and Dr. Irina O. Petruseva (ICBFM SB RAS, Novosibirsk, Russia), respectively. The AP-CSD YB-1 mutant was a kind gift from Lev P. Ovchinnikov and Dmitry Kretov. NAD+ and β-nicotinamide mononucleotide were from Sigma (USA), [α-³²P]ATP and [γ-³²P]ATP were from ICBFM SB RAS. Olaparib was from Selleckchem.