Tecnai g2 sphera microscope

Manufactured by Thermo Fisher Scientific

The Tecnai G2 Sphera is a high-performance transmission electron microscope (TEM) designed for advanced imaging and analysis. It features a spherical aberration corrector, enabling improved resolution and contrast for material science and life science applications.

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Lab products found in correlation

Laceycarbon tem grids, by Electron Microscopy Sciences (1 mentions) Digital micrograph software, by Ametek (1 mentions) Ultrascan 1000 uhs ccd camera, by Ametek (1 mentions) Jsm 6510lv microscope, by JEOL (1 mentions)

Close spelling variants of this product

Tecnai G2 (325 citations) Tecnai G2 Sphera (15 citations) G2 Sphera (3 citations) Tecnai Sphera (2 citations) Sphera microscope (2 citations)

3 protocols using tecnai g2 sphera microscope

Cryo-TEM Sample Preparation Protocol

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Cryo-TEM samples were prepared
by freshly glow discharging 400 mesh
holey carbon TEM grids (Quantifoil R2/2, 400 mesh Cu support) or lacey
carbon TEM grids (Electron Microscopy Sciences, Hatfield, PA) using
an Emitech K350 glow discharge unit and plasma-cleaned for 90 s in
an E.A. Fischione 1020 unit. A 4 μL portion of sample was applied
to each grid, gently blotted to form a thin film, and then rapidly
plunged into liquid ethane. The grids were transferred to liquid nitrogen
and then imaged on an FEI Tecnai G2 Sphera microscope operating at
200 keV. The samples were kept <175 °C during imaging. Micrographs
were recorded on a 2k × 2k Gatan CCD camera.

Zang N., Issa J.B., Ditri T.B., Bortone D.S., Touve M.A., Rush A.M., Scanziani M., Dombeck D.A, & Gianneschi N.C. (2020). Multicolor Polymeric Nanoparticle Neuronal Tracers. ACS Central Science, 6(3), 436-445.

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Nanodisc Visualization by Electron Microscopy

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Purified nanodiscs were diluted to ~10μ g/ml and 3μl of the diluted sample was applied on to a glow-discharged carbon grid. The protein sample was absorbed for 30 seconds and blotted away. The grid was then washed and stained with 2% uranyl acetate. The grids were left air dry and loaded into a Tecnai G2 Sphera microscope (FEI) equipped with a LaB6 electron gun and a Gatan Ultrascan 1000 UHS CCD camera (Gatan, Inc.) for analysis. Images of nanodiscs were taken at a voltage of 200KV and a magnification of 65,000× in Digital Micrograph software (Gatan, Inc.).

Zhang P., Ye F., Bastidas A.C., Kornev A.P., Wu J., Ginsberg M.H, & Taylor S.S. (2015). An Isoform specific Myristylation Switch Targets Type II PKA Holoenzymes to Membranes. Structure (London, England : 1993), 23(9), 1563-1572.

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Morphological Analysis of Materials

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The morphology of the materials was studied by transmission electron microscopy (TEM) and by scanning electron microscopy (SEM). The TEM images were recorded on a Tecnai G2 Sphera microscope (FEI) at an acceleration voltage of 120 kV using a LaB6 cathode. The samples were prepared by placing 5 µL of solution on the plasma treated carbon mesh and by removing the excess liquid after 2 min. The obtained mesh with the material was installed on the sample holder and placed in the microscope.
For SEM imaging, 20 µL dispersion was deposited on the surface of an adhesive tape previously placed on the sample holder. After the evaporation of the solution, the sample holder with the remaining material was gold coated. All the SEM images were obtained on a JSM-6510LV microscope (Jeol) with an acceleration voltage of 20 kV.

Pavlovic M., Rouster P, & Szilagyi I. (2017). Synthesis and formulation of functional bionanomaterials with superoxide dismutase activity. Nanoscale, 9(1).

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