F4 80 monoclonal antibody bm8

Manufactured by Thermo Fisher Scientific

The F4/80 Monoclonal Antibody (BM8) is a laboratory reagent used in the identification and study of macrophages. It recognizes the F4/80 antigen, a glycoprotein expressed on the surface of murine macrophages. This antibody can be used in various applications, such as flow cytometry, immunohistochemistry, and cell sorting, to detect and isolate macrophage populations.

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Lab products found in correlation

Biotin, by Thermo Fisher Scientific (1 mentions) Pstat1 tyr701 9167, by Cell Signaling Technology (1 mentions) P akt ser473 4060, by Cell Signaling Technology (1 mentions) Akt1 2967, by Cell Signaling Technology (1 mentions) P gsk 3β 5558, by Cell Signaling Technology (1 mentions) Alexa conjugated antibody, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole dapi sc 3598, by Santa Cruz Biotechnology (1 mentions) Recombinant mouse ifn γ, by Thermo Fisher Scientific (1 mentions) Mouse serum albumin, by Merck Group (1 mentions) Lithium chloride (licl), by Merck Group (1 mentions) Doxorubicin, by Pfizer (1 mentions)

2 protocols using f4 80 monoclonal antibody bm8

Comprehensive Immunoblotting Protocol

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2Antibodies against GAPDH sc-322 (Santa Cruz Biotechnology, Santa Cruz, CA), pSTAT1 Tyr701 #9167, pS6 #15967, pAkt Ser473 #4060, Akt1 #2967, pGSK3β #5558, pH2AX (γ-H2AX) mAb #9718, pp53 #9286, CHK1 # 2348 and pBRCA1 #9009 were obtained from Cell Signaling Technology (Danvers, MA). F4/80 Monoclonal Antibody (BM8) coupled to Biotin (eBioscience, Waltham, MA) was used according to manufacturer's instructions. p-Histone H3, ab32107 was obtained from Abcam (Cambridge, MA). Alexa conjugated antibodies were obtained from Thermo Fisher Scientific (Waltham, MA). AffiniPure goat and rabbit anti-horseradish peroxidase (HRP) were obtained from Jackson Immunoresearch (San Diego, CA) and 4′,6-Diamidino-2-phenylindole (DAPI) SC-3598 from Santa Cruz Biotechnology (Santa Cruz, CA). Recombinant mouse IFNγ (PeproTech, Rocky Hill, NJ) was dissolved in 0.002% mouse serum albumin (MSA; Sigma-Aldrich, St. Louis, MO) and used at 2.5 μg/kg of weight. LiCl (Sigma-Aldrich, St Louis, MO) was used at 4 mg/mice and 4 μM in vitro. Rapamycin used as previously reported by us [14] (link) Doxorubicin was obtained from Pfizer and used at 3.45 μM. AZD8055 and XAV939 were used as previously reported by us [16] (link), [17] . Dextran sulfate sodium YD318041799 (Carbosynth, San Diego, CA) was dissolved at 3% in drinking water.

Gutiérrez-Martínez I.Z., Rubio J.F., Piedra-Quintero Z.L., Lopez-Mendez O., Serrano C., Reyes-Maldonado E., Salinas-Lara C., Betanzos A., Shibayama M., Silva-Olivares A., Candelario-Martinez A., Meraz-Ríos M.A., Schnoor M., Villegas-Sepúlveda N, & Nava P. (2018). mTORC1 Prevents Epithelial Damage During Inflammation and Inhibits Colitis-Associated Colorectal Cancer Development. Translational Oncology, 12(1), 24-35.

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Quantifying Hepatic Macrophage Infiltration

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To quantify and characterize the hepatic inflammatory cell infiltrate, immunohistochemistry for F4/80⁺ (F4/80 IHC) cells was performed on formaldehyde (4% neutral‐buffered)‐fixed, paraffin‐embedded liver sections using a monoclonal mouse antibody. Sections were deparaffinated, rehydrated and digested with 0.1% protease. Endogenous peroxidase was blocked with 1% H₂O₂ in methanol. Specific binding of the F4/80 antibody was detected using a biotinylated anti‐rat IgG and the ABC‐System with amino‐9‐ethyl‐carbazole as substrate.³¹ Antibody details: F4/80 Monoclonal Antibody (BM8), eBioscience™, catalog # 14‐4801‐82, Thermo Fisher Scientific.

Fuchs C.D., Krivanec S., Steinacher D., Mlitz V., Wahlström A., Stahlman M., Claudel T., Scharnagl H., Stojakovic T., Marschall H, & Trauner M. (2020). Absence of Bsep/Abcb11 attenuates MCD diet‐induced hepatic steatosis but aggravates inflammation in mice. Liver International, 40(6), 1366-1377.

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