Anti vps35

Anti-M6PR (cation independent) antibody [2G11] (ab2733; Abcam).
Anti-APP (A8717; Sigma).
Anti-APP 6e10 to detect Aβ, sAPPα (SIG-39320; Covance).
Anti-GAPDH (Sigma).
Anti-tubulin (Sigma).
Anti-cyclophilin B (ACB0825; Atgen).
Anti-sAPPβ Swedish (6A-1; IBL).
Anti-VPS35 (SC-374372; Santa Cruz).
Anti-LAMP-1 (SC-18821; Santa Cruz).
Anti-PLD3 (HPA012800; Sigma).
Anti-transferrin receptor (13-6800; Invitrogen).
Anti-SORL1 (612633; BD Bioscience).
Anti-GFP (Seaman lab).
Anti-LBPA (MABT837; Sigma).
Anti-EEA1 (610456; BD Biosciences).
Anti-GM130 (610822; BD Biosciences).
Anti-TGN46 (Seaman lab).
Anti-MICALL1 (H00085377-B01P, Novus).
Anti-SNAP29 (gift from Andrew Peden, University of Sheffield, UK).
Anti-PACSIN2 (ab37615, Abcam).

Most general reagents used in this study were sourced from Sigma-Aldrich. The siRNA oligonucleotides were purchased from Dharmacon. Primary antibodies used in this study were as follows: anti-TBC1D5 [Santa Cruz, sc-376296, dilution 1:400 or 1:1000 for immunofluorescence (IF) microscopy or western blotting (WB), respectively], anti-VPS26 (Abcam, ab23892, 1:800 IF or 1:1000 WB), anti-VPS35 [Santa Cruz, sc-374372, 1:800 IF or 1:1000 WB, or from the Seaman lab (see Seaman, 2007 (link)), 1:300 for IF], anti-CIMPR (Abcam, ab2733, 1:400 IF or 1:1000 WB), anti-Lamp1 (Santa Cruz, sc-18821, 1:500 IF or 1:1000 WB), anti-Glut1 (Abcam, ab15309, 1:400 IF), anti-GM130 (BD Transduction labs 610822, 1:500 IF), anti-Fam21 (Millipore, ABT79, 1:400 IF or 1:1000 WB), anti-Aβ (Covance, SIG-39320, 1:1000 WB), anti-sAPPβ (IBL America, 10321, 1:800 WB), anti-Rab7a:GTP (NewEast Biosciences, 26923, 1:300 IF), anti-TGN46 (Seaman lab, see Seaman, 2007 (link), dilution 1:600 IF), anti-GFP (Seaman lab, see Seaman et al., 2009 (link), 1:1000 for immunoprecipitation), anti-Snx1 (BD Transduction labs, dilution 1:400 IF or 1:1000 WB) and anti-Tubulin (Sigma-Aldrich, dilution 1:1000 WB). Secondary fluorescently labelled antibodies were purchased from Invitrogen.