Protein was isolated from cells as described21 (link). Briefly, cell lysates were electrophoresed on 4-20% Pierce Precise Protein Gels (Thermo Scientific) and transferred to nitrocellulose membranes. Membranes were probed with the following primary antibodies: rabbit-anti-ATG16L1 (1:500, Sigma) guineapig-anti-p62 (1:1000, Progen Biotechnik, Heidelberg, Germany), rabbit anti-Caspase-1 (1:5000, Dr. Gabriel Nuñez), rat anti-Caspase-11 (1:250, Sigma), rat anti-NLRP3 (1:250, R&D systems), and rabbit-anti-GAPDH (1:1000, Cell Signaling Technology, Danvers, MA). Goat-anti-rabbit IgG-HRP, goat-anti-guinea pig IgG-HRP, and goat-anti-rat IgG-HRP secondaries (Santa Cruz Biotechnology, Dallas, TX) were used and detected by SuperSignal West Dura (Thermo Scientific).
Rat anti caspase 11
Manufactured by Merck Group
Sourced in Germany
Rat anti-Caspase-11 is a laboratory reagent that can be used to detect the presence of Caspase-11 protein in samples. Caspase-11 is an enzyme involved in the inflammatory response and cell death pathways. This product can be used in various research applications that require the identification or quantification of Caspase-11.
Automatically generated - may contain errors
Lab products found in correlation
Supersignal west dura, by Thermo Fisher Scientific (2 mentions)
Pierce precise protein gels, by Thermo Fisher Scientific (2 mentions)
Rabbit anti atg16l1, by Merck Group (2 mentions)
Guinea pig anti p62, by Progen Biotechnik (2 mentions)
Rabbit anti caspase 1, by Merck Group (2 mentions)
Rat anti nlrp3, by R&D Systems (2 mentions)
Goat anti guinea pig igg hrp, by Santa Cruz Biotechnology (2 mentions)
Goat anti rat igg hrp, by Santa Cruz Biotechnology (2 mentions)
Goat anti rabbit igg hrp, by Santa Cruz Biotechnology (2 mentions)
Rabbit anti gapdh, by Cell Signaling Technology (2 mentions)
2 protocols using rat anti caspase 11
1
Western Blot Analysis of Autophagy Markers
2
Western Blot Analysis of Autophagy Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Protein was isolated from cells as described21 (link). Briefly, cell lysates were electrophoresed on 4-20% Pierce Precise Protein Gels (Thermo Scientific) and transferred to nitrocellulose membranes. Membranes were probed with the following primary antibodies: rabbit-anti-ATG16L1 (1:500, Sigma) guineapig-anti-p62 (1:1000, Progen Biotechnik, Heidelberg, Germany), rabbit anti-Caspase-1 (1:5000, Dr. Gabriel Nuñez), rat anti-Caspase-11 (1:250, Sigma), rat anti-NLRP3 (1:250, R&D systems), and rabbit-anti-GAPDH (1:1000, Cell Signaling Technology, Danvers, MA). Goat-anti-rabbit IgG-HRP, goat-anti-guinea pig IgG-HRP, and goat-anti-rat IgG-HRP secondaries (Santa Cruz Biotechnology, Dallas, TX) were used and detected by SuperSignal West Dura (Thermo Scientific).
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