Nanozoomer digital pathology viewer software

Per FFPE tissue block, one 10 μm section and three 4 μm sections were sliced, mounted on silane-coated slides and dried overnight at 37 °C. The 10 μm FFPE tissue sections were deparaffinized (10 min xylene) and imaged with the NIR fluorescence flatbed. Afterwards, the 10 μm tissue sections and the subsequent 4 μm tissue sections were stained with hematoxylin and eosin (H&E). The H&E slides were digitalized by the Nanozoomer 2.0-HT slide scanner (Hamamatsu) and viewed with use of NanoZoomer Digital Pathology (NDP) viewer software (Hamamatsu). Blinded for the fluorescence signals and under supervision of an experienced gastrointestinal pathologist (A.K.), different tissue areas were selected: low-grade dysplasia (LGD) areas within the adenoma, normal adjacent tissue within the adenoma section and normal colon crypts within the normal tissue biopsies. Afterwards, these areas were superimposed on the NIR fluorescence Odyssey images. Ex vivo analyses of the snap frozen tissue was shown to be unreliable, as bevacizumab-800CW signals diminished during thawing of the samples.