BEC (99%), DDAB (98%), BAC12 (puriss, ≥99 %), BAC14 (puriss, ≥99 %), BAC16 (99%), hexadecyltrimethylammonium bromide (HDTAB, 99.5%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). HDTAB was used as an internal standard (ISTD) for BEC and DDAB. Deuterated ISTDs (BAC12-d5, BAC14-d5, and BAC16-d5, 99%) were purchased from CDN Isotopes Inc. (Pointe Claire, Quebec). LCMS-grade acetonitrile, 2-propanol, formic acid, and ammonium formate were purchased from Thermo Scientific (Waltham, MA, USA). Water (18 MΩ) was cleaned by a Millipore Milli-Q system (Billerica, MA, USA). Polypropylene centrifuge tubes (15 ml) were purchased from Fisher Scientific. All samples and standards were passed through regenerated cellulose membrane filters (0.2-μm pore size, 15-mm i.d., Phenomenex, Torrance, CA, USA).
Bac12
Manufactured by Merck Group
Sourced in United States
BAC12 is a piece of laboratory equipment designed for general use in research and analytical settings. It serves as a basic centrifuge for the separation of materials based on density differences.
Automatically generated - may contain errors
Lab products found in correlation
Formic acid, by Thermo Fisher Scientific (3 mentions)
Bac14, by Merck Group (2 mentions)
Bac16, by Merck Group (2 mentions)
Polypropylene centrifuge tubes, by Thermo Fisher Scientific (1 mentions)
Ammonium formate, by Thermo Fisher Scientific (1 mentions)
Regenerated cellulose membrane filters, by Phenomenex (1 mentions)
Lc ms grade acetonitrile, by Thermo Fisher Scientific (1 mentions)
2 propanol, by Thermo Fisher Scientific (1 mentions)
Milli q system, by Merck Group (1 mentions)
Hexadecyltrimethylammonium bromide, by Merck Group (1 mentions)
Isopropanol, by Avantor (1 mentions)
Ethyl acetate, by Thermo Fisher Scientific (1 mentions)
Hplc grade acetonitrile acn, by Honeywell (1 mentions)
Hplc grade formic acid, by Avantor (1 mentions)
Milli q water purification system, by Merck Group (1 mentions)
3 protocols using bac12
1
Quantitative Analysis of Cationic Surfactants
2
Antimicrobial Efficacy Evaluation Protocol
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Stationary phase cultures were diluted to a final cell concentration of 107 cfu/ml in a 96-well plate with wells holding a final reaction volume of 200 μl of fresh medium containing different concentrations of the investigated antimicrobial substance. The tested concentrations of BAC (Sigma-Aldrich, 234427), benzyl-dimethyl-dodecylammonium chloride (BAC12; Sigma-Aldrich, 13380) and NBD-DDA were 10, 20, 30, 40, 60, 100, and 150 μM. The plates were incubated at 37°C with agitation for 24 h and growth was assessed visually. To determine the MBC (minimum bactericidal concentration), 10 μl from each well without visible growth were spotted onto LB agar medium and incubated at 37°C for 24 h. The first concentration that reduced the initial cell density by a factor of 103 or more was designated the MBC. NBD-DDA was dissolved in DMSO. To assess the effect of DMSO on growth, a MIC (minimum inhibitory concentration) assay with the solvent DMSO alone was conducted. Growth was not inhibited by DMSO in the concentration range tested here. All experiments were conducted with n = 3 biological replicates and yielded the same MIC and MBC.
3
Analytical Characterization of Quaternary Ammonium Compounds
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Water was obtained from a Milli-Q water purification system (Millipore). HPLC-grade acetonitrile (ACN) was purchased from Honeywell. HPLC-grade ammonium acetate, acetone, and ethyl acetate were purchased from Thermo Fisher Scientific. Isopropanol and HPLC-grade formic acid were purchased from VWR. Standard mixtures of C12–C14 alkyl (ethyl benzyl) dimethyl ammonium chloride (61% AEB-12, 25% AEB-14; ≥98.0%) and benzalkonium chloride 10% solution (1.79% BAC-10, 67.83% BAC-12, 24.6% BAC-14, 4.54% BAC-16, 1.24% BAC-18) were purchased from Sigma Aldrich. An individual BAC-10 standard was purchased from Toronto Research Chemicals. An additional proprietary compound (X), best described as a hydroxylated QAC containing a long-chained hydrocarbon and an inorganic component, was obtained from the manufacturer and included in testing.
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