Histone h4 sc 25260

Cell culture media and reagents were purchased from Gibco (Carlsbad, CA). Manganese (II) chloride (MnCl₂), protease inhibitor cocktail, poly-L-lysine, and dimethyl sulfoxide (DMSO) were purchased from MilliporeSigma (St. Louis, MO). Antibodies for EAAT2 (ab41621), phosphoserine (ab9332) and goat anti-rabbit IgG (ab97051) were obtained from Abcam (Cambridge, MA). Antibodies for YY1 (sc-7341), HDAC1 (sc-81598), HDAC3 (sc-376957), β-actin (sc-47778), and histone H4 (sc-25260) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). ChIP-validated EAAT2 primers and biotinylated oligonucleotides were obtained from Invitrogen (Carlsbad, CA). The AurkB inhibitor Hesperadin (Hesp) and the CK2 inhibitor CX-4945 (CX) were purchased from Cayman Chemical (Ann Arbor, MI). All chemicals were prepared in phosphate-buffered saline (PBS), double-distilled H₂O or DMSO and diluted to working concentrations in Opti-MEM prior to use.

Cell culture media and reagents were purchased from Gibco (Carlsbad, CA). Manganese (II) chloride (MnCl2), N-Acetyl-L-cysteine (NAC), α-tocopherol (AT), protease inhibitor cocktail, and dimethyl sulfoxide (DMSO) were purchased from MilliporeSigma (St. Louis, MO). The TNF-α inhibitor (E)-4-2-4-chloro-3-nitrophenyl (C-87) and the IKK-β inhibitor IKK16 were purchased from Tocris (Littleton, CO). The chloromethyl derivative of 2′,7′-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) was purchased from Life Technologies, Inc. (Carlsbad, CA). Antibodies for EAAT2 (ab41621), IKK-β (ab32135) and phospho-IKK-β (ab59195) were obtained from Abcam (Cambridge, MA); antibodies for NF-κB p65 (sc-8008), YY1 (sc-7341), β-actin (sc-47778), and histone H4 (sc-25260) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Bright-Glo luciferase reporter assay kits were purchased from Promega (Madison, WI), and an RNA isolation kit was obtained from Qiagen (Valencia, CA). The TNF-α standard tetramethylbenzidine (TMB) enzyme-linked immunosorbent assay (ELISA) development kit for human samples (900-T25) was purchased from PeproTech (Rocky Hill, NJ). All chemicals were prepared in phosphate-buffered saline (PBS), double-distilled H2O or DMSO, according to the manufacturer’s instructions, and diluted to working concentration in Opti-MEM prior to use.