One to two microliters of the sample aliquots was mixed with 0.5 μl of matrix mix (Sigma-Aldrich) on a steel target. The mass spectra were recorded on an UltrafleXtreme MALDI-TOF/TOF mass spectrometer (Bruker Daltonics) equipped with a neodymium laser. The molecular MH+ ions were measured in reflector mode; the accuracy of the measured results was within 0.1 Da.
Matrix mix
Manufactured by Merck Group
Matrix mix is a lab equipment product designed for mixing and homogenizing samples. It provides a consistent and reliable method for preparing samples for further analysis or processing.
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3 protocols using matrix mix
1
MALDI-TOF Protein Analysis Protocol
2
MALDI-TOF/TOF Analysis of Biomolecules
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Sample aliquots were combined with the matrix mix (Sigma-Aldrich) on a steel target. The mass spectra were recorded on an UltrafleXtreme MALDI-tandem time of flight (TOF/TOF) mass spectrometer (Bruker Daltonics) equipped with a neodymium laser. The molecular MH+ ions were measured in reflector mode; the accuracy of the measured results was within 0.1 Da.
3
High-Resolution Mass Spectrometry of Biomolecules
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High resolution electrospray ionization mass spectrometry (ESI-MS) analysis. Q-TOF Maxis Impact II (Bruker Daltonics) mass-spectrometer with electrospray ionization was used for sample analysis. Lyophilized HPLC fractions from biochemical reactions were dissolved in 0.1% formic acid in deionized water and introduced directly to the instrument using the syringe pump.
The spectra were recorded in a positive ion mode in a range from 100 m/z to 750 m/z. The temperature of ion source was 200 ºC, pressure of carrier gas 2.5 bar, the gas flow 5 ml/min, the voltage at the capillary 4 kV. The fragmentation spectra were recorded in AutoMS mode.
Matrix Assisted Laser Desorption Time of Flight (MALDI-TOF) mass spectrometry.
Sample aliquots were combined with the matrix mix (Sigma-Aldrich) on a steel target. The mass spectra were recorded on an UltrafleXtreme MALDI-TOF/TOF mass spectrometer (Bruker Daltonics) equipped with a neodymium laser. The molecular MH+ ions were measured in reflector mode; the accuracy of the measured results was within 0.1 Da.
The spectra were recorded in a positive ion mode in a range from 100 m/z to 750 m/z. The temperature of ion source was 200 ºC, pressure of carrier gas 2.5 bar, the gas flow 5 ml/min, the voltage at the capillary 4 kV. The fragmentation spectra were recorded in AutoMS mode.
Matrix Assisted Laser Desorption Time of Flight (MALDI-TOF) mass spectrometry.
Sample aliquots were combined with the matrix mix (Sigma-Aldrich) on a steel target. The mass spectra were recorded on an UltrafleXtreme MALDI-TOF/TOF mass spectrometer (Bruker Daltonics) equipped with a neodymium laser. The molecular MH+ ions were measured in reflector mode; the accuracy of the measured results was within 0.1 Da.
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