For anatomical analyses, shoot apices and young leaves were isolated, dehydrated through a tertiary butyl alcohol series [62 ], embedded in Paraplast® (Leica Microsystems Inc., Heidelberg, Germany), and serial-sectioned at a 12 µm thickness on a Leica RM2145 rotary microtome. Longitudinal and transverse sections were stained with astra blue and safranin O [64 ] and the slides were mounted with resin Permount (Fisher Scientific, Pittsburgh, PA, USA). Observations and photographs were performed using a Leica DMLB light microscope.
Sigma vp scanning electron microscope
The Sigma VP Scanning Electron Microscope is a versatile instrument designed for high-resolution imaging and analysis of a wide range of materials. It features a stable electron column, advanced detectors, and a user-friendly interface, enabling researchers to obtain detailed information about the surface morphology and composition of their samples.
Lab products found in correlation
18 protocols using sigma vp scanning electron microscope
Micromorphology and Anatomy of Plant Nectaries
For anatomical analyses, shoot apices and young leaves were isolated, dehydrated through a tertiary butyl alcohol series [62 ], embedded in Paraplast® (Leica Microsystems Inc., Heidelberg, Germany), and serial-sectioned at a 12 µm thickness on a Leica RM2145 rotary microtome. Longitudinal and transverse sections were stained with astra blue and safranin O [64 ] and the slides were mounted with resin Permount (Fisher Scientific, Pittsburgh, PA, USA). Observations and photographs were performed using a Leica DMLB light microscope.
Characterization of CNF and TOCNF Hydrogels
High-Resolution Volumetric Imaging of Neuronal Ultrastructure
Micromorphology and Anatomy of Plant Nectaries
For anatomical analyses, shoot apices and young leaves were isolated, dehydrated through a tertiary butyl alcohol series [62 ], embedded in Paraplast® (Leica Microsystems Inc., Heidelberg, Germany), and serial-sectioned at a 12 µm thickness on a Leica RM2145 rotary microtome. Longitudinal and transverse sections were stained with astra blue and safranin O [64 ] and the slides were mounted with resin Permount (Fisher Scientific, Pittsburgh, PA, USA). Observations and photographs were performed using a Leica DMLB light microscope.
Ultrastructural Analysis of Axonal Swelling
Correlating SEM with DAPI-Stained Cells
Wax Suspension SEM Imaging
wax suspension was
dropped on silica and dried overnight. A Au/Pd coating of 4 nm was
sputtered on the sample before observation with a Zeiss Sigma VP scanning
electron microscope (SEM) operating at 2 kV. Several images were taken,
and the most representative one is shown.
Aerogel Sample Preparation for SEM Imaging
SEM images were acquired with a Zeiss Sigma VP scanning electron microscope with an acceleration voltage of 1.0 to 1.5 kV. The aerogel sample was placed on the carbon tape and sputter-coated with Au/Pd with Emitech K950X/K350 or a Leica EM ACE600 high vacuum sputter coater. Sample preparation methods, coatings, and acceleration voltages for each matrix appear in Supplementary Table
Spheroid Analysis: Imaging and Kinetics
Ultrastructural Analysis of Dendritic Spines
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