Primary antibody against cd206

Soybean phosphatidylcholine (S100) was purchased from Lipoid GmbH (Ludwigshafen, Germany). Cholesterol, 1,2-distearoyl-sn-glycero-3-phosphoethanolamine- N-[amino(polyethylene glycol)-2000] (DSPE-PEG₂₀₀₀) and DSPE-PEG₂₀₀₀-N-hydroxysuccinimide ester (NHS) were purchased from A.V.T. Pharmaceutical Co., Ltd. (Shanghai, China). 4-Aminophenyl α-D-mannopyranoside was obtained from J&K Scientific Ltd. (Beijing, China). Coumarin-6 was purchased from Sigma-Aldrich (Saint Louis, MO, USA). 4′,6-Diamidino-2-phenylindole (DAPI) and LysoTracker Red were purchased from Beyotime (Haimen, Jiangsu, China). APC-CD86 and PE-CD206 were supplied by BioLegend (San Diego, CA, USA). The primary antibody against CD206 was obtained from Abcam (Cambridge, MA, USA). The primers of Arg1, Mgl2, and Ym1 were obtained from Invitrogen (Carlsbad, CA, USA). Recombinant murine interleukin-4 (IL-4) was supplied by PeproTech (Rocky Hill, NJ, USA). Dulbecco’s Modified Eagle’s Medium (DMEM) and fetal bovine serum (FBS) were purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). Temozolomide capsules were purchased from Jiangsu Tasly Diyi Pharmaceutical Co., Ltd. (Huai’an, Jiangsu, China). All other organic reagents were of analytical grade and purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).