Cells were obtained from ATCC and fingerprinted as in Barretina et al (Barretina et al., 2012 (link)). Cells were maintained in RPMI-1640 (NCI-H1299, HCC364, NCI-H1975, and HCC827; Corning, Corning, NY), McCoy’s 5A (CALU1; Gibco, Waltham, MA) or DMEM (MGH-065; Invitrogen, Carlsbad, CA) supplemented with 2 mM glutamine, 50 U/mL penicillin, 50 U/mL of streptomycin (Gibco), and 10% fetal bovine serum (Sigma, St. Louis, MO), and incubated at 37°C in 5% CO2. MGH-065 cells were derived as previously described (Crystal et al., 2014 (link)). Cell lines were tested for mycoplasma prior to screening. Trametinib, vemurafenib, erlotinib, and afatinib, were purchased from Selleck Chemicals (Houston, TX). LDK-378 and Crizotinib were synthesized by the Novartis Global Discovery Chemistry Department.
Nci h1299
Manufactured by Corning
Sourced in United States
NCI-H1299 is a human cell line derived from a non-small cell lung carcinoma. It is commonly used in laboratory research as a model system for studying lung cancer. The cell line maintains the essential characteristics of the original tumor and is a valuable tool for investigating cellular and molecular mechanisms related to lung cancer.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640, by Corning (2 mentions)
Afatinib, by Selleck Chemicals (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Erlotinib, by Selleck Chemicals (1 mentions)
Trametinib, by Selleck Chemicals (1 mentions)
Hcc827, by Corning (1 mentions)
Nci h1975, by Corning (1 mentions)
Vemurafenib, by Selleck Chemicals (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Beas 2b, by Corning (1 mentions)
Lipofectamine 2000 reagent, by Thermo Fisher Scientific (1 mentions)
Mg132, by BD (1 mentions)
Matrigel, by BD (1 mentions)
Penicillin streptomycin, by Solarbio (1 mentions)
Hek293t, by Corning (1 mentions)
3 protocols using nci h1299
1
Cell Line Authentication and Maintenance
2
Cell Culture and Exosome Depletion Protocol
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All cell lines, including NCI-H1299, A549, SPC-A-1, LTEP-A2, and Beas-2B were purchased from Cell Bank/Stem Cell Bank (Shanghai, China). The human normal lung epithelial cell line Beas-2B was cultured in DMEM basal medium (Corning, USA). The human NSCLC cell lines NCI-H1299, A549, SPC-A-1, and LTEP-A2 were cultured using RPMI-1640 basal medium (Corning, USA). The above basal culture medium was supplemented with 10% fetal bovine serum (FBS; PAN, Germany), 100-μg/mL penicillin, and 100-U/mL streptomycin (New Cell & Molecular Biotech, USA). Cells were cultured in a humidified incubator at 37 °C with 5% CO2. Exosomes were removed from FBS at 110,000 × g, 4 °C, for 8 h by an ultracentrifuge device (BECKMAN COULTER, USA) as previously described. The Lipofectamine 2000 reagent (Invitrogen, USA) was used to deliver the recombinant plasmids into cells as previously reported [2 (link)].
3
Evaluating triptolide's effects on TP53-null lung cancer
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Human lung epithelial NCI-H1299 (TP53-null) and HEK-293T cells were purchased from Shanghai Fuheng Biological (Shanghai, China). All cell lines were authenticated by short tandem repeat (STR) profiling identification. New cell batches were thawed every 3–4 months for use in these experiments. NCI-H1299 cells were maintained in RPMI-1640 (Corning Inc., Corning, NY, USA) medium, and HEK-293T cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM; Corning). Both media contained 10% fetal bovine serum (FBS; Corning) and 1% penicillin/streptomycin (Solarbio, Beijing, China), and all cells were maintained at 37 ℃ in a humidified chamber with 5% CO2 and passaged twice weekly. The agent triptolide was purchased from MCE (New Jersey, USA) and used at a concentration of 30 nM (the dose selection of triptolide refers to the previous experimental research in this laboratory (31 (link)). After pre-experiment, the drug concentration and drug action time of 30 nmol, which has no killing effect on cells and has the most obvious effect, are selected). MG132 was used at a concentration of 20 µM, and nutlin-3A was used at a concentration of 20 µM. Matrigel was purchased from BD (356234, Franklin Lakes, NJ, USA).
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