Ab204306

After deparaffinization and blocking, the sections were incubated with primary anti-HNF1A rabbit polyclonal antibody (ab204306; Abcam, Cambridge, UK) at a dilution of 1:200 overnight at 4 °C. The sections were incubated with biotinylated secondary antibody (Vectastain Universal Elite; Vector Laboratories, Burlingame, CA, USA) for 30 min, and signals were detected using the ImmPACT DAB kit (Vector Laboratories) with 5-min incubation. All sections were counterstained with hematoxylin.

Cultured cells were fixed with 4% formaldehyde and blocked. They were incubated with primary anti-HNF1A rabbit antibody (ab204306; Abcam, Cambridge, UK) at a dilution of 1:200 and anti-POU5F1 mouse antibody (#75463; Cell Signaling Technology, Beverly, MA) at a dilution of 1:200 overnight at 4 °C. Cells were incubated with secondary antibody (Goat anti-Rabbit IgG Secondary Antibody Alexa Fluor 488; A11008, Thermo Fisher Scientific, Waltham, MA, USA, Goat anti-Mouse IgG Secondary Antibody Alexa Fluor 594; A11005, Thermo Fisher Scientific) at a dilution of 1:2000 for 90 min. The side was mounted in Prolong Gold with DAPI (Thermo Fisher Scientific) overnight.