Interleukin 6 il 6

6-G (purity, ≥95%) was purchased from Sigma Chemical Co. (MO, USA). Antibodies PI3K, Akt, p-Akt, LY294002 (LY), and β-tubulin were supplied by Cell Signaling Technology (MA, USA). TNF-a, IL-6, IL-1β, NLRP3, and caspase-1 were provided by Abcam (Cambridge, UK). 2,3,5-triphenyltetrazolium chloride (TTC), BCA protein quantification kit, and the second antibody were purchased from Beyotime (Changsha, China). Cardiac troponin T (cTnT), creatine kinase-MB (CK-MB), tumor necrosis factor-a (TNF-a), interleukin-6 (IL-6), and interleukin-1β (IL-1β) enzyme-linked immunosorbent assay (ELISA) were acquired from Cusabio Biotech Co. (MD, USA).

The level of interleukin 1 beta (IL-1β), interleukin 6 (IL- 6), and nuclear factor-kappa B (NF-κB) was determined using an enzyme-linked immunosorbent assay (ELISA) kit. Rat NF-κB ELISA Kit and Interleukin 6 (IL-6) were purchased from CUSABIO, Inc., Wuhan, Hubei, China. Rat interleukin 1 beta (IL-1β), ELISA Kit was purchased from MyBioSource, San Diego, US.