The galantamine hydrobromide analysis was studied by using Waters 1525 HPLC system with Waters 2489 UV/VIS detector. The isocratic HPLC system consists of Agilent Eclipse XDB RF C18 column (5 μm, 4.6 × 150 mm), with trifluoroacetic acid/water/acetonitrile (0.01/85/15 v/v) as the mobile phase. The mobile phase was delivered in the flow rate of 1 mL/min and the wavelength chosen for galantamine hydrobromide detection was 290 nm. The standard calibration curve was plotted in concentration range of 100 ppm to 1000 ppm. The calibration curve showed linear relationship between the peak area and the concentration of galantamine hydrobromide solution, with R2 > 0.99.
Eclipse xdb rf c18 column
Manufactured by Agilent Technologies
The Eclipse XDB RF C18 column is a high-performance liquid chromatography (HPLC) column designed for the separation and analysis of a wide range of compounds. It features a bonded C18 stationary phase, providing a stable and reproducible platform for reversed-phase chromatography. The column is suitable for a variety of applications, including pharmaceutical, environmental, and food analysis.
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2 protocols using eclipse xdb rf c18 column
1
HPLC Analysis of Galantamine Hydrobromide
2
HPLC Analysis of Growth Hormone Release
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Samples withdrawn from Franz diffusion cells were analyzed by using Waters 1525 HPLC system along with Waters 2489 UV/Vis detector. The isocratic, reversed phase HPLC system consists of Agilent Eclipse XDB RF C18 column (5 μm, 4.6×150 mm). A mixture of trifluoroacetic acid/water/acetonitrile (0.01/85/15 v/v) was used as the mobile phase, with a flow rate of 1 mL/min. The wavelength was set at 290 nm for GH detection. GH release was calculated according to the standard calibration curve with a line equation (y=884.86x−16,100) and correlation coefficient (R2=0.9956) in the range of 100–1,000 mg/L.
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