Isoflurane florene

Paraffin (Paraplast Tissue Embedding Medium REF 501006) was purchased from McCormick Scientific (St. Louis, MO) and hematoxylin from Merck (Germany). Hypoxyprobe-^TM1-Kit was obtained from HPI (Burlington, USA). Protein block and polyclonal rabbit anti-mouse immunoglobulins were from DAKO (Denmark). H₂O₂ was from Roth (Germany), Tween 20 from Sigma-Aldrich (Germany), ABC kit from Vector Laboratories (Burlington, USA), 3′,3′-diaminobenzidine (DAB) from Thermo Fisher Scientific (Fremont, USA), and mouse anti-HIF-1α antibody from Novus Biologicals (Cambridge, UK). Isoflurane (Florene) was obtained from Abbott (Wiesbaden, Germany), ketamine 10% from Ceva (Düsseldorf, Germany), lidocaine (Xylocain 1%) from AstraZeneca (Wedel, Germany), and Ringer's solution Macoflex N from MacoPharma International (Langen, Germany). Portex catheters (0.58 mm i.d., 0.96 mm o.d.) were purchased from Smiths Medical International (Hythe, UK) and medical oxygen was from Air Liquide (Düsseldorf, Germany).

Hydrogen peroxide, and o-dianisidine were obtained from Sigma-Aldrich (St. Louis, MO) and formalin solution (10%, buffered) and hematoxylin 51260 from Fluka/Sigma-Aldrich (Steinheim, Germany). Isoflurane (Florene) was from Abbott (Wiesbaden, Germany), ketamine 10% from Ceva (Düsseldorf, Germany), lidocaine (Xylocain 1%) from AstraZeneca (Wedel, Germany), and Ringer’s solution Macoflex N from MacoPharma International (Langen, Germany). Portex catheters (0.58 mm inner diameter, 0.96 mm outer diameter) were supplied by Smiths Medical International (Hythe, U.K.). Paraffin was purchased from (Paraplast Tissue Embedding Medium REF 501006) McCormick Scientific (St. Louis, MO), and medical oxygen from Air Liquide (Düsseldorf, Germany).