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Anti hibadh

Manufactured by Proteintech
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Anti-HIBADH is a highly specific antibody that recognizes the HIBADH protein. HIBADH, or 3-hydroxyisobutyrate dehydrogenase, is an enzyme involved in the catabolism of valine. This antibody can be used to detect and quantify HIBADH in various biological samples.

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Lab products found in correlation

Calcimycin, by Merck Group (2 mentions) Activated charcoal, by Merck Group (2 mentions) 2 4 dinitrophenol, by Merck Group (2 mentions) Anti p erk1 2 thr202 tyr204, by Cell Signaling Technology (2 mentions) Anti fatp3, by Proteintech (2 mentions) Anti fatp4, by Abnova (2 mentions) Anti pkc θ, by BD (2 mentions) Anti occludin 1, by Abcam (2 mentions) Anti calnexin, by Thermo Fisher Scientific (2 mentions) Sulfo n succinimidyl oleate, by Santa Cruz Biotechnology (2 mentions) Recombinant vegfa, by R&D Systems (2 mentions) Vegf b, by R&D Systems (2 mentions) Anti pan actin, by Cell Signaling Technology (2 mentions) Anti akt, by Cell Signaling Technology (2 mentions) 2 deoxyglucose, by Merck Group (2 mentions) Anti erk1 2, by Cell Signaling Technology (2 mentions) Anti p akt ser473, by Cell Signaling Technology (2 mentions) Mitotracker red cmxros, by Cell Signaling Technology (2 mentions) Ecl kit, by Thermo Fisher Scientific (2 mentions) Anti β actin monoclonal antibody, by Merck Group (1 mentions)

Close spelling variants of this product

Anti-TM

3 protocols using anti hibadh

1

Western Blot and Immunostaining Techniques

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Anti-Pan-Actin (#4968), Anti-ERK1/2 (#9102), anti-pERK1/2 Thr 202/Tyr204 (#9101), anti-AKT (#4685), anti-pAKT Ser473 (#9271 all from Cell Signaling), anti-FATP3 (Proteintech 12943-1-AP), anti-FATP4 (Abnova H00010999-M01), anti-PKC-θ (BD Transduction lab 610089), anti-Na,K ATPase A1 (Novus Biologicals NB300-146SS) and anti-HIBADH (Proteintech 13466-1-AP) antibodies were used for Western blot (1:1000 dilution). Anti-Occludin-1 (Abcam ab31721) and anti-Calnexin (Thermo MA3-27) antibodies were used for immunostaining (1:200 dilution). Mitotracker® Red CMXRos (Cell Signaling 9082S) was used for mitochondria staining. Sulfo-N-succinimidyl Oleate (sc-208408) was purchased from Santa Cruz. Akt VIII (#124018) was purchased from Calbiochem. CHC (#5029) was purchased from Tocris. Recombinant VEGFA (#293-VE-010) and VEGFB (#767-VE-010) were purchased from R&D systems. Human insulin (Humulin R U-100) was purchased from Harvard Drug Group (#821501). Membrane filters (MWCO 3 kDa #Z677094), Calcimycin (#C9275), 2,4-Dinitrophenol (#D198501), 2-deoxyglucose (#D8375), activated charcoal (#C4386) and other chemicals were purchased from Sigma unless otherwise stated.
Jang C., Oh S.F., Wada S., Rowe G.C., Liu L., Chan M.C., Rhee J., Hoshino A., Kim B., Ibrahim A., Baca L.G., Kim E., Ghosh C.C., Parikh S.M., Jiang A., Chu Q., Forman D.E., Lecker S.H., Krishnaiah S., Rabinowitz J.D., Weljie A.M., Baur J.A., Kasper D.L, & Arany Z. (2016). A branched chain amino acid metabolite drives vascular transport of fat and causes insulin resistance. Nature medicine, 22(4), 421-426.
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2

Western Blot and Immunostaining Techniques

Check if the same lab product or an alternative is used in the 5 most similar protocols
Anti-Pan-Actin (#4968), Anti-ERK1/2 (#9102), anti-pERK1/2 Thr 202/Tyr204 (#9101), anti-AKT (#4685), anti-pAKT Ser473 (#9271 all from Cell Signaling), anti-FATP3 (Proteintech 12943-1-AP), anti-FATP4 (Abnova H00010999-M01), anti-PKC-θ (BD Transduction lab 610089), anti-Na,K ATPase A1 (Novus Biologicals NB300-146SS) and anti-HIBADH (Proteintech 13466-1-AP) antibodies were used for Western blot (1:1000 dilution). Anti-Occludin-1 (Abcam ab31721) and anti-Calnexin (Thermo MA3-27) antibodies were used for immunostaining (1:200 dilution). Mitotracker® Red CMXRos (Cell Signaling 9082S) was used for mitochondria staining. Sulfo-N-succinimidyl Oleate (sc-208408) was purchased from Santa Cruz. Akt VIII (#124018) was purchased from Calbiochem. CHC (#5029) was purchased from Tocris. Recombinant VEGFA (#293-VE-010) and VEGFB (#767-VE-010) were purchased from R&D systems. Human insulin (Humulin R U-100) was purchased from Harvard Drug Group (#821501). Membrane filters (MWCO 3 kDa #Z677094), Calcimycin (#C9275), 2,4-Dinitrophenol (#D198501), 2-deoxyglucose (#D8375), activated charcoal (#C4386) and other chemicals were purchased from Sigma unless otherwise stated.
Jang C., Oh S.F., Wada S., Rowe G.C., Liu L., Chan M.C., Rhee J., Hoshino A., Kim B., Ibrahim A., Baca L.G., Kim E., Ghosh C.C., Parikh S.M., Jiang A., Chu Q., Forman D.E., Lecker S.H., Krishnaiah S., Rabinowitz J.D., Weljie A.M., Baur J.A., Kasper D.L, & Arany Z. (2016). A branched chain amino acid metabolite drives vascular transport of fat and causes insulin resistance. Nature medicine, 22(4), 421-426.
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3

Western Blot Analysis of Hepatic Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Equal aliquots of total proteins were loaded and separated on 4–12% NuPAGE Bis-Tris gels (Invitrogen, Cartsbad, CA) and then electrotransferred onto nitrocellulose membranes. The polyclonal anti-CA3, anti-BHMT, anti-HIBADH, anti-ECHS1, and anti-ACOX1 antibodies were purchased from Proteintech (Chicago, IL); anti-PPARα and anti-NRF2 polyclonal antibodies were obtained from Santa Cruz (Santa Cruz, CA). The monospecific anti-CYP2E1 antibody was a gift from Dr. Jerome Lasker, Hackensack Biomedical Research Institute, Hackensack, NJ. The monoclonal anti-β actin antibody was purchased from Sigma-Aldrich (St. Louis, MO). Secondary antibodies conjugated with horseradish peroxidase were used to amplify the immuno-recognition signals with the peroxidase activity monitored using an ECL kit (Thermo Scientific, Pittsburgh, PA). The images were captured with FluorChem Q (ProteinSimple, San Jose, CA).
Wang Y., Kou Y., Wang X., Cederbaum A, & Wang R. (2014). Multifactorial Comparative Proteomic Study of Cytochrome P450 2E1 Function in Chronic Alcohol Administration. PLoS ONE, 9(3), e92504.
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