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Nanosem 230 microscope

Manufactured by Thermo Fisher Scientific
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The NanoSEM 230 is a scanning electron microscope designed for high-resolution imaging of nanoscale samples. It provides detailed topographical and compositional information about the surface of a specimen.

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FEI Nova NanoSEM 230 microscope Nova NanoSEM 230 scanning electron microscope Nova NanoSEM 230 microscope NanoSEM 230

2 protocols using nanosem 230 microscope

1

SEM Imaging of GNP-Bound Extracellular Vesicles

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SEM images of GNP binding to EVs were generated using EVs purified from 50 μL of human plasma with ExoQuick kits to reduce SEM artifacts. Purified EVs were immobilized on sensor chips and hybridized with anti-CD63-AuS and anti-CD9-AuR, as described above. Sensor chips were then PBS (pH 7.0) washed, dried under a gentle N2 stream, then treated with a direct current sputter coating approach to apply a 3nm layer of iridium and imaged with a NOVA NanoSEM 230 microscope (FEI) at a 5kV acceleration voltage in high vacuum (3 × 10−6 Torr) using a 5 mm working distance. Three randomly selected 80–120 μm2 SEM fields were analyzed to calculate the number of total and GNP-bound EVs per μm2 for each assay.
Liang K., Liu F., Fan J., Sun D., Liu C., Lyon C.J., Bernard D.W., Li Y., Yokoi K., Katz M.H., Koay E.J., Zhao Z, & Hu Y. (2017). Nanoplasmonic Quantification of Tumor-derived Extracellular Vesicles in Plasma Microsamples for Diagnosis and Treatment Monitoring. Nature biomedical engineering, 1, 0021.
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2

SEM Imaging of GNP-Bound Extracellular Vesicles

Check if the same lab product or an alternative is used in the 5 most similar protocols
SEM images of GNP binding to EVs were generated using EVs purified from 50 μL of human plasma with ExoQuick kits to reduce SEM artifacts. Purified EVs were immobilized on sensor chips and hybridized with anti-CD63-AuS and anti-CD9-AuR, as described above. Sensor chips were then PBS (pH 7.0) washed, dried under a gentle N2 stream, then treated with a direct current sputter coating approach to apply a 3nm layer of iridium and imaged with a NOVA NanoSEM 230 microscope (FEI) at a 5kV acceleration voltage in high vacuum (3 × 10−6 Torr) using a 5 mm working distance. Three randomly selected 80–120 μm2 SEM fields were analyzed to calculate the number of total and GNP-bound EVs per μm2 for each assay.
Liang K., Liu F., Fan J., Sun D., Liu C., Lyon C.J., Bernard D.W., Li Y., Yokoi K., Katz M.H., Koay E.J., Zhao Z, & Hu Y. (2017). Nanoplasmonic Quantification of Tumor-derived Extracellular Vesicles in Plasma Microsamples for Diagnosis and Treatment Monitoring. Nature biomedical engineering, 1, 0021.
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