Phospho plc γ1 tyr783

Manufactured by Cell Signaling Technology

Sourced in Germany

Phospho-PLC-γ1-Tyr783 is a research-use antibody that recognizes the tyrosine 783 phosphorylation site on phospholipase C gamma 1 (PLC-γ1). PLC-γ1 is an important enzyme involved in signal transduction pathways.

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Lab products found in correlation

Anti fas igm antibody, by Merck Group (2 mentions) Phospho zap70 tyr319, by Cell Signaling Technology (2 mentions) Anti phospho lat tyr132, by Abcam (2 mentions) Anti lck, by Santa Cruz Biotechnology (2 mentions) Phospho akt ser473, by Cell Signaling Technology (2 mentions) Anti plcγ, by Santa Cruz Biotechnology (2 mentions) β actin, by Cell Signaling Technology (2 mentions) Phospho lat tyr171, by Cell Signaling Technology (2 mentions) Anti human cd3 okt3 monoclonal antibody, by Thermo Fisher Scientific (2 mentions) Anti erk, by Santa Cruz Biotechnology (1 mentions) Anti human cd3 okt3, by Thermo Fisher Scientific (1 mentions) Anti human cd28 cd28.2, by BD (1 mentions) Anti 6 his hrp, by Abcam (1 mentions) Phospho mek ser221, by Cell Signaling Technology (1 mentions) Phospho lat tyr191, by Cell Signaling Technology (1 mentions) Anti grb2, by Abcam (1 mentions) Anti mek, by Cell Signaling Technology (1 mentions) N n 3 5 difluorophenacetyl l alanyl s phenylglycine t butyl ester dapt, by Merck Group (1 mentions) Losartan, by Merck Group (1 mentions) Wortmannin, by Merck Group (1 mentions)

7 protocols using phospho plc γ1 tyr783

T Cell Activation Signaling Pathway

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The anti-Fas (IgM) antibody, anti-phospho-LAT-Tyr226, and rabbit polyclonal anti-human LAT were from Merck-Millipore; anti-PLC-γ1 mAb, anti-Lck, and anti-Erk were from Santa Cruz Biotechnology (Santa Cruz, CA, USA); antibodies binding phospho-Erk, phospho-PLC-γ1-Tyr783, phospho-ZAP70-Tyr319, phospho-MEK-Ser221, phospho-LAT-Tyr191, and anti-MEK were from Cell Signaling Technology; anti-6His-HRP, anti-Grb2, and anti-phospho-LAT-Tyr132 antibodies were from Abcam (Cambridge, MA, USA); and anti-CD69-APC (allophycocyanin) and anti-β-actin monoclonal antibodies were from Biolegend. The protein synthesis inhibitor cicloheximide was purchased from Merck-Millipore. Stimulations were performed with anti-human CD3 (OKT3; eBioscience) or anti-human CD28 (CD28.2; BD Pharmingen) monoclonal antibodies.

Arbulo-Echevarria M.M., Narbona-Sánchez I., Fernandez-Ponce C.M., Vico-Barranco I., Rueda-Ygueravide M.D., Dustin M.L., Miazek A., Duran-Ruiz M.C., García-Cózar F, & Aguado E. (2018). A Stretch of Negatively Charged Amino Acids of Linker for Activation of T-Cell Adaptor Has a Dual Role in T-Cell Antigen Receptor Intracellular Signaling. Frontiers in Immunology, 9, 115.

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Angiotensin II Signaling in Cardiovascular Cells

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Recombinant rat PDGF was purchased from R&D Systems. Human Ang II, losartan, sunitinib, wortmannin, and DAPT (N‐[N‐(3,5‐Difluorophenacetyl)‐L‐alanyl]‐S‐phenylglycine t‐butyl ester) were purchased from Sigma‐Aldrich. Primary antibodies of phospho‐Akt Ser473, phospho‐Akt Thr308, Akt, phospho‐PLCγ1 Tyr783, PLCγ1, 3‐phosphoinositide‐dependent protein kinase‐1 (PDK1), mammalian target of rapamycin (mTOR), and Notch2 were purchased from Cell Signaling Technology; Hey2 antibody was purchased from Beijing Biosynthesis Biotechnology; antibody of Notch1 used in Western blot was from Santa Cruz Biotechnology; and Notch1 intracellular domain (N1‐ICD) antibody used in immunohistochemistry was from Millipore.

Jiang D., Zhuang J., Peng W., Lu Y., Liu H., Zhao Q., Chi C., Li X., Zhu G., Xu X., Yan C., Xu Y., Ge J, & Pang J. (2017). Phospholipase Cγ1 Mediates Intima Formation Through Akt‐Notch1 Signaling Independent of the Phospholipase Activity. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 6(7), e005537.

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Western Blot Analysis of HER2 Signaling

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All cells were lysed in RIPA buffer (Beyotime) supplemented with protease and phosphatase inhibitors (Roche). Following lysis, the cells were sonicated. Western blotting was performed using standard methods. The blots were probed with the following antibodies: phospho-HER2-Tyr1221/1222 (Epitomics), β-actin (Sigma), phospho-HER2-Tyr877, phospho- HER2-Tyr1248(p-HER2), HER2, PLCγ1, phospho-PLCγ1-Tyr783, PARP, STAT5, phospho- STAT5-Tyr694, AKT, phospho-AKT-Ser473 (pAKT), ERK1/2, phospho-ERK1/2-Thr202/Tyr204, S6, phospho-S6-Ser235/236 (pS6) and phosphor-4E-BP1-Thr37/46 (Cell Signaling Technology).

Hu Z., Wan X., Hao R., Zhang H., Li L., Li L., Xie Q., Wang P., Gao Y., Chen S., Wei M., Luan Z., Zhang A., Huang N, & Chen L. (2015). Phosphorylation of Mutationally Introduced Tyrosine in the Activation Loop of HER2 Confers Gain-of-Function Activity. PLoS ONE, 10(4), e0123623.

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T-cell Activation Signaling Pathway

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Phorbol 12-myristate 13-acetate (PMA) and ionomycin (were purchased from Sigma-Aldrich (St. Louis, MO, USA) and were prepared in DMSO. Anti-LAT, anti-Lck, anti-PLC-γ, and phospho-Erk antibodies were from Santa Cruz Biotechnology (Heidelberg, Germany); antibodies binding ZAP-70, phospho-ZAP-70-Tyr319, β-actin, phospho-PLC-γ1-Tyr783, and phospho-LAT-Tyr171 were from Cell Signaling Technology (Danvers, MA, USA); anti-Grb2 mAb was from Abcam (Cambridge, MA, USA); and anti-Erk mAb was from BioLegend (San Diego, CA, USA). Stimulations were performed with the anti-human CD3 OKT3 monoclonal antibody (eBioscience, Thermo Fisher Scientific). Staining to assess CD3 expression on the membrane was performed with OKT3 antibody conjugated to APC (BioLegend, San Diego, CA, USA).

Vico-Barranco I., Arbulo-Echevarria M.M., Serrano-García I., Pérez-Linaza A., Miranda-Sayago J.M., Miazek A., Narbona-Sánchez I, & Aguado E. (2021). A Novel, LAT/Lck Double Deficient T Cell Subline J.CaM1.7 for Combined Analysis of Early TCR Signaling. Cells, 10(2), 343.

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T-cell Signaling Pathway Analysis

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The anti-Fas (IgM) antibody was from Merck-Millipore; anti-LAT LAT-01 mAb was from EXBIO (Praha, Czech Republic); anti-LAT 11B.12, anti-PLC- γ, anti-PTP1B, and anti-caspase-3 monoclonal antibodies were from Santa Cruz Biotechnology (Heidelberg, Germany); antibodies binding phospho-Erk, β-actin, phospho-PLC-γ1-Tyr783 and phospho-LAT-Tyr171 were from Cell Signaling Technology; anti-6His-HRP antibody and anti-phospho-LAT-Tyr132 were from Abcam (Cambridge, United Kingdom). The protein synthesis inhibitor cycloheximide was purchased from Merck-Millipore. Stimulations were performed with the anti-human CD3 OKT3 monoclonal antibody (eBioscience).

Arbulo-Echevarria M.M., Vico-Barranco I., Narbona-Sánchez I., García-Cózar F., Miazek A, & Aguado E. (2020). Increased Protein Stability and Interleukin-2 Production of a LATG131D Variant With Possible Implications for T Cell Anergy. Frontiers in Cell and Developmental Biology, 8, 561503.

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Signaling Pathway Protein Analysis

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Western blot assays were carried out using established protocols and probed with the following antibodies: phospho-Akt (Ser473) 1:1000 (Cell Signaling 9271), Total Akt 1:1000 (Cell Signaling 9272), phospho-MEK1/2 1:5000 (Cell Signaling 9154), Total MEK1/2 1:5000 (Cell Signaling 9122), p44/42 MAPK (Erk1/2) 1:5000 (Cell Signaling 9101), Total MAPK 1:5000 (Cell Signaling 9102), phospho-FGF Receptor (Tyr653/654) 1:500 (Cell Signaling 3471), FGF Receptor 2 (D4L2V) 1:500 (Cell Signaling 23328), phospho-PLCγ1 (Tyr783) 1:1000 (Cell Signaling 14008), PLCγ1 (D9H10) 1:1000 (Cell Signaling 5690), phospho-FRS2-α (Tyr196) 1:1000 (Cell Signaling 3864), FRS2 1:1000 (abcam 10425), phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) 1:1000 (Cell Signaling 4228), PI3 Kinase p85 (19H8) 1:000 (Cell Signaling 4257), β-actin 1:10000 (Cell Signaling 4967).

Krook M.A., Bonneville R., Chen H.Z., Reeser J.W., Wing M.R., Martin D.M., Smith A.M., Dao T., Samorodnitsky E., Paruchuri A., Miya J., Baker K.R., Yu L., Timmers C., Dittmar K., Freud A.G., Allenby P, & Roychowdhury S. (2019). Tumor heterogeneity and acquired drug resistance in FGFR2-fusion-positive cholangiocarcinoma through rapid research autopsy. Cold Spring Harbor Molecular Case Studies, 5(4), a004002.

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Antibody Immunostaining Protocol

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Mouse monoclonal antibodies to GM130 and Golgin97 were from Transduction Laboratories and Molecular Probes, respectively. Sheep anti-TGN46 was purchased from Serotech and mouse anti-PentaHis was from Qiagen. Antibodies against PLCγ1 and phospho-PLCγ1 (Tyr783) were purchased from Cell Signalling. Rabbit polyclonal anti-GFP, anti-FLAG and anti-ectodomain of VSV-G were purchased from Sigma-Aldrich. Cyanine 3-conjugated mouse and rabbit secondary antibodies were from Jackson ImmunoResearch Laboratories and Alexa Fluor 488, 546 and 647-conjugated anti-mouse, anti-rabbit and anti-sheep antibodies were obtained from Molecular Probes. Peroxidase-conjugated secondary antibodies were from Promega.

Sicart A., Katan M., Egea G, & Sarri E. (2015). PLCγ1 participates in protein transport and diacylglycerol production triggered by cargo arrival at the Golgi. Traffic (Copenhagen, Denmark), 16(3).

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