Anti mnk1

The following antibodies (Abs) and reagents were used in present studies: anti-IL-1β (human specific, mouse specific, and human & mouse specific), anti-phospho NF-κB, anti-NF-κB, anti-phospho ERK, anti-ERK, anti-phospho p38, anti-p38, anti-phospho 4E-BP1, anti-4E-BP1, anti-phospho Mnk1, anti-Mnk1, anti-phospho eIF4E, anti-eIF4E, anti-phospho MK2, anti-MK2, and anti-streptavidin-HRP antibodies were purchased from cell signaling technology (Danvers, MA). Anti-β-actin, Dimethyl sulfoxide, Actinomycin D, Cyclohexamide, ultrapure E. coli O111:B4 LPS, Rapamycin, PD98059, and SB202190 were purchased from Sigma-Aldrich (St. Louis, MO). Anti-NLRP3 antibody was purchased from Adipogen (San Diego, CA). Torin 1 and FR180204 were purchased from Merck Millipore (Billerica, MA). CGP57380 and MK25 were purchased from TOCRIS (Ellisville, MO, USA) and Cayman Chemical (Ann Arbor, MI), respectively. Recombinant human M-CSF and mouse M-CSF were purchased from R&D system (Minneapolis, MN).

Bilirubin (B4126-5G) was purchased from Sigma-Aldrich (USA). Vemurafenib (S1267), KO-947 (S8569), and LY3214996 (S8534) were obtained from Selleckchem (Houston, TX). Antibodies, including anti-PARP (#9532), Caspase 3 (#9662), anti-cleaved Caspase-3 (Asp175) (#9661), anti-Caspase 9 (#9502), anti-cleaved Caspase-9 (Asp315) (#9505), anti-phospho-B-Raf (Ser445) (#2696), anti-B-Raf (#14814), anti-phospho-MEK1/2 (Ser217/221) (#3958), anti-MEK1/2 (#4694), anti-phospho-ERK1/2 (Thr202/Tyr204) (#4370), anti-ERK1/2 (#4695), anti-phospho-MNK1 (Thr197/202) (#2111), anti-MNK1 (#2195), anti-phospho-eIF4E (Ser209) (#9741), anti-eIF4E (#2067), anti-phospho-p70S6K (Thr421/Ser424) (#9204), anti-p70S6K (#2708), anti-phospho-4eBP1(Thr37/46) (#2855), anti-4eBP1 (#9644), and anti-GAPDH (#5174) were purchased from Cell Signaling Technology (Beverly, MA).