Tumor tissue samples from 8–10 FFPE slide-mounted sections were manually dissected, with corresponding H&E sections used to guide dissections, to obtain at least 70% tumor purity. RNA was isolated by using the Maxwell system (Promega). RNA was quantitated by fluorescence dye staining by using the Quant-iT (Life Technologies) RNA assay. RNA quality was evaluated by using a 2100 Bioanalyzer (Agilent Technologies) with a Nano RNA 6000 Chip. RNASEQ libraries enriched for coding regions were prepared by using the Truseq RNA Access Library Prep Kit (Illumina), following the manufacturer’s protocol for RNA input quantity relative to RNA quality. Sequencing was performed on HiSeq2000 (Illumina) to generate 100-bp paired-end reads.
Nano rna 6000 chip
Manufactured by Illumina
The Nano RNA 6000 Chip is a lab equipment product designed to analyze and quantify RNA samples. It uses microfluidic technology to assess the quality and quantity of RNA in a small sample volume. The chip can handle a range of RNA concentrations and supports the detection of small, fragmented RNA molecules.
Automatically generated - may contain errors
2 protocols using nano rna 6000 chip
1
FFPE Tumor RNA Extraction and Sequencing
2
FFPE Tumor RNA Extraction and Sequencing
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