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Target protein hbsag

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Target protein HBsAg is a surface antigen of the hepatitis B virus (HBV). It is a key component in the diagnosis, monitoring, and management of hepatitis B infection.

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5 protocols using target protein hbsag

1

Lentiviral Expression of Anti-HBsAg scFv

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Example 79

Cells are cultured in the presence of a lentivirus encoding the exogenous transgene antibody scFv against hepatitis B surface antigen (HBsAg) (SEQ ID No. 1) such that the final cell product expresses >1×10^5 copies of antibody scFv receiver per cell as assessed by flow cytometry. To ensure that the receiver is functionally expressed, in vitro activity is assessed by flow cytometry. Briefly, cells are incubated with target protein HBsAg (Abcam) that is directly labeled with Dylight 650 fluorophore at a range of concentrations. Incubated cells are washed and analyzed by flow cytometry for presence of the fluorophore.

US10329531B2. Synthetic membrane-receiver complexes (2019-06-25). RUBIUS THERAPEUTICS, INC. [US]. Inventors: Avak Kahvejian [US], Jordi Mata-Fink [US], John Round [US], David Arthur Berry [US], Noubar B. Afeyan [US].
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2

Lentiviral-mediated Hepatitis B Antigen Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 79

Cells are cultured in the presence of a lentivirus encoding the exogenous transgene antibody scFv against hepatitis B surface antigen (HBsAg) (SEQ ID No. 1) such that the final cell product expresses >1×10{circumflex over ( )}5 copies of antibody scFv receiver per cell as assessed by flow cytometry. To ensure that the receiver is functionally expressed, in vitro activity is assessed by flow cytometry. Briefly, cells are incubated with target protein HBsAg (Abcam) that is directly labeled with Dylight 650 fluorophore at a range of concentrations. Incubated cells are washed and analyzed by flow cytometry for presence of the fluorophore.

US10557119B2. Erythroid cells comprising phenylalanine ammonia lyase (2020-02-11). RUBIUS THERAPEUTICS, INC. [US]. Inventors: Avak Kahvejian [US], Jordi Mata-Fink [US], John Round [US], David Arthur Berry [US], Noubar B. Afeyan [US].
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3

Lentivirus-Mediated Antibody Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 79

Cells are cultured in the presence of a lentivirus encoding the exogenous transgene antibody scFv against hepatitis B surface antigen (HBsAg) (SEQ ID No. 1) such that the final cell product expresses >1×10^5 copies of antibody scFv receiver per cell as assessed by flow cytometry. To ensure that the receiver is functionally expressed, in vitro activity is assessed by flow cytometry. Briefly, cells are incubated with target protein HBsAg (Abcam) that is directly labeled with Dylight 650 fluorophore at a range of concentrations. Incubated cells are washed and analyzed by flow cytometry for presence of the fluorophore.

US10301594B1. Synthetic membrane-receiver complexes (2019-05-28). RUBIUS THERAPEUTICS, INC. [US]. Inventors: Avak Kahvejian [US], Jordi Mata-Fink [US], John Round [US], David Arthur Berry [US], Noubar B. Afeyan [US].
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4

Lentiviral-mediated Hepatitis B Antigen Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 79

Cells are cultured in the presence of a lentivirus encoding the exogenous transgene antibody scFv against hepatitis B surface antigen (HBsAg) (SEQ ID No. 1) such that the final cell product expresses >1×10{circumflex over ( )}5 copies of antibody scFv receiver per cell as assessed by flow cytometry. To ensure that the receiver is functionally expressed, in vitro activity is assessed by flow cytometry. Briefly, cells are incubated with target protein HBsAg (Abcam) that is directly labeled with Dylight 650 fluorophore at a range of concentrations. Incubated cells are washed and analyzed by flow cytometry for presence of the fluorophore.

US10344263B2. Synthetic membrane-receiver complexes (2019-07-09). RUBIUS THERAPEUTICS, INC. [US]. Inventors: Avak Kahvejian [US], Jordi Mata-Fink [US], John Round [US], David Arthur Berry [US], Noubar B. Afeyan [US].
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5

Lentivirus-Mediated Antibody Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 79

Cells are cultured in the presence of a lentivirus encoding the exogenous transgene antibody scFv against hepatitis B surface antigen (HBsAg) (SEQ ID No. 1) such that the final cell product expresses >1×10^5 copies of antibody scFv receiver per cell as assessed by flow cytometry. To ensure that the receiver is functionally expressed, in vitro activity is assessed by flow cytometry. Briefly, cells are incubated with target protein HBsAg (Abcam) that is directly labeled with Dylight 650 fluorophore at a range of concentrations. Incubated cells are washed and analyzed by flow cytometry for presence of the fluorophore.

US10253296B2. Synthetic membrane-receiver complexes (2019-04-09). RUBIUS THERAPEUTICS, INC. [US]. Inventors: Avak Kahvejian [US], Jordi Mata-Fink [US], John Round [US], David Arthur Berry [US], Noubar B. Afeyan [US].
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