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Ubp 310

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The UBP 310 is a laboratory equipment designed for protein purification. It features a compact and robust design to facilitate efficient and reliable protein extraction and purification processes.

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Lab products found in correlation

Polyethyleneimine solution, by Merck Group (1 mentions) B27 supplement, by Thermo Fisher Scientific (1 mentions) Trypsin 2.5, by Thermo Fisher Scientific (1 mentions) Bicuculline, by Cayman Chemical (1 mentions) Ethylene glycol tetraacetic acid (egta), by AppliChem (1 mentions) Hepes, by Dia-M (1 mentions) Neurobasal a medium, by PanEco (1 mentions) Ethylene diamine tetraacetic acid (edta), by AppliChem (1 mentions) Fura 2 am, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Merck Group (1 mentions) D ap5, by Alomone (1 mentions) L ap4, by Bio-Techne (1 mentions) Stereotaxic apparatus, by Kopf Instruments (1 mentions)

4 protocols using ubp 310

1

Neuronal Cell Culture Reagents

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The reagents that were used in the experiments are listed below. (1) Sigma-Aldrich, Saint Louis, MO, USA: Poly(ethyleneimine) solution (Cat. no. P3143), penicillin–streptomycin (Cat. no. P4333), L-Glutamine (Cat. No G85402). (2) Life Technologies, Grand Island, NY, USA: B-27 supplement (Cat. no. 17504044), Trypsin 2.5% (Cat. no. 15090046). (3) Molecular Probes, Eugene, OR, USA: Fura-2 AM (Cat. no. F1221). (4) Tocris Bioscience, Bristol, UK: UBP 310 (Cat. no. 3621), 5-Nonyloxytryptamine oxalate (Cat. No. 0901), WIN 55,212-2 mesylate (Cat. no. 1038) (5) Alomone Labs, Jerusalem, Israel: D-AP5 (Cat. no. D-145), NBQX (Cat. no. N-185). (6) Cayman Chemical, Ann Arbor, MI, USA: Bicuculline (Cat. no. 11727), HEMADO (Cat. No. 21015); (7) AppliChem, Darmstadt, Germany: EDTA (Cat. no. A5097), EGTA (Cat. no. A-0878). (8) Dia-M, Moscow, Russian Federation: HEPES (Cat. no. 3350). (9) Abcam, Cambridge, UK: N6-Cyclohexyladenosine (Cat. no. ab120472). (10) Paneco, Moscow, Russian Federation: Neurobasal-A medium.
Laryushkin D.P., Maiorov S.A., Zinchenko V.P., Mal’tseva V.N., Gaidin S.G, & Kosenkov A.M. (2023). Of the Mechanisms of Paroxysmal Depolarization Shifts: Generation and Maintenance of Bicuculline-Induced Paroxysmal Activity in Rat Hippocampal Cell Cultures. International Journal of Molecular Sciences, 24(13), 10991.
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2

Pharmacological Dissection of Retinal Circuits

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The following chemicals, all purchased from Sigma-Aldrich (St. Louis, MO), were added either alone or in combination to the Ames medium and applied to the entire retina via the superfusion: the glycine receptor antagonist strychnine (1 µM, S8753, Sigma), the GABAA receptor antagonist SR-95531 (GABAzine; 5 µM, S106, Sigma), the GABAC receptor antagonist (1,2,5,6-tetrahydropyridin-4-yl) methylphosphinic acid (TPMPA; 50 µM, T200, Sigma), the NMDA receptor antagonist, D-(−)2-amino-5-phosphonopetanoic acid (D-AP5; 50 µM, #0106, Tocris Bioscience, Minneapolis, MN), the AMPA receptor antagonist 2,3-dioxo-6-nitro-l,2,3,4-tetrahydrobenzo[f]quinoxaline-7-sulfonamide disodium salt (NBQX; 10 µM #1044, Tocris Bioscience) and the GLUK5 kainate receptor antagonist (S)-l-(2-amino-2-carboxyethyl)-3-(2-carboxy-thiophene-3-yl-methyl)-5-methylpyrimidine-2,4-dione (UBP 310, 10 µm #3621, Tocris Bioscience). Chemicals were maintained in separate oxygenated flasks and were delivered to the retinal superfusion chamber with a 6 channel valve controller (Warner Instruments, Hamden, CT, VC6) via a single manifold; due to the relatively large size of the incubation chamber needed to hold the primate retina wash-in and wash-out of the antagonist solution required ~2 min of superfusion.
Crook J.D., Packer O.S, & Dacey D.M. (2014). A synaptic signature for ON- and OFF-center parasol ganglion cells of the primate retina. Visual neuroscience, 31(1), 57-84.
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3

Selective Bipolar Cell Signaling

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We blocked ON-bipolar cell signaling via application of the mGluR6 agonist L-AP4 (20 µM; Tocris, part 0103). We blocked photoreceptor to OFF-bipolar cell signaling via application of the kainate receptor antagonist, UBP 310 (50 µM; Tocris, part 3621).
Tiriac A., Smith B.E, & Feller M.B. (2018). Light prior to eye-opening promotes retinal waves and eye-specific segregation. Neuron, 100(5), 1059-1065.e4.
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4

Intracerebral Drug Delivery in Rodent Models

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Animals were anesthetized with a mixture of 8.7 mg/ml ketamine (Provet) and 2 mg/ml xylazil (Provet) and placed in a stereotaxic apparatus (Kopf Instruments). Osmotic micropumps (Model 1007D; Alzet) were filled with 0.8834 μg/μl (5.3 μg total daily dose) of UBP310 (Tocris) or vehicle control (10% DMSO) and implanted subcutaneously along the back of the neck. An infusion cannula (PlasticsOne) connected to the micropump was placed in the right lateral ventricle at AP -0.2, ML +1.0, DV -2.8 relative to bregma for all MPTP and drug measurement studies. For all 6-OHDA studies the infusion cannula was placed in the left lateral ventricle at AP -0.2, ML -1.0, DV -2.8 to ensure cannula did not impede subsequent lesioning and the pump was removed 1 week later and the cannula tubing sealed with heat. All stereotaxic coordinates were based on the Paxinos atlas for the mouse brain (Paxinos et al. 2001) .
Stayte S., Laloli K.J., Rentsch P., Lowth A., Li K.M., Pickford R, & Vissel B. (2020). The kainate receptor antagonist UBP310 but not single deletion of GluK1, GluK2, or GluK3 subunits, inhibits MPTP-induced degeneration in the mouse midbrain. Experimental neurology, 323.
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