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2 azobis isobutyronitrile aibn

Manufactured by Merck Group

2-Azobis(isobutyronitrile) (AIBN) is a chemical compound commonly used as a free radical initiator in various applications. It is a white, crystalline solid with the molecular formula C8H12N4. AIBN decomposes to generate free radicals, which can then initiate various polymerization reactions or other chemical processes.

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2 protocols using 2 azobis isobutyronitrile aibn

1

Dasatinib-Loaded Polymer Nanoparticles

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Dasatinib and DOX·HCl were purchased from LC Laboratories. 4-nitrophenyl chloroformate was purchased from Acros. 2-Azobis(isobutyronitrile) (AIBN) was purchased from Sigma-Aldrich and purified by recrystallization in anhydrous ethanol. Dulbecco’s phosphate buffered saline (DPBS) was purchased from Lonza. Penicillin-streptomycin solution and fetal bovine serum (FBS) were purchased from Invitrogen. Dulbecco’s Modified Eagle’s Medium (DMEM), trypsin-EDTA solution, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), Triton X-100, Hoechst 33342 and LysoTracker Green DND-26 were all purchased from Sigma-Aldrich. N-methacryloyl-N-(tert-butoxycarbonyl)aminohexyl methacrylamide (MBA) [39 (link)], N-methacryloyl-N′-(t-butyoxycarbonyl)cystamine (MBC) [40 (link)], POEG macroCTA [34 (link)], and 4-nitrophenyl-actived Dasatinib Das-NO2 [41 (link)] were prepared according to the literatures. All other reagents were of analytical or chromatographic grade.
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2

Synthesis and Characterization of Multivalent Cysteine Conjugates

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DOX·HCl was purchased from LC Laboratories. 4-Nitrophenyl chloroformate was purchased from Acros. Dulbecco's modified Eagle's medium (DMEM), trypsin-EDTA solution, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), Triton X-100 and Hoechst 33342 were purchased from Sigma-Aldrich. Penicillin-streptomycin solution and fetal bovine serum (FBS) were purchased from Invitrogen. 2-Azobis(isobutyronitrile) (AIBN, Sigma-Aldrich) was purified by recrystallization in anhydrous ethanol22 (link). N,N′-(t-butyoxycarbonyl)cystamine (MBC)23 (link) and POEG macroCTA24 (link) were prepared according to the literature. All other reagents were of analytical or chromatographic grade.
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