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Ferrous sulfate feso4

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Ferrous sulfate (FeSO4) is an inorganic compound that is commonly used in laboratory settings. It consists of iron (Fe) and sulfate (SO4) ions. Ferrous sulfate is a green crystalline solid that is soluble in water. Its core function is to serve as a source of iron in various chemical reactions and analyses.

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Lab products found in correlation

Hydrogen peroxide h2o2, by Merck Group (3 mentions) Ammonium thiocyanate, by Merck Group (2 mentions) Inulin, by Merck Group (2 mentions) Melatonin, by Merck Group (1 mentions) Lpo 586 kit for lpo, by Enzo Life Sciences (1 mentions) Indole 3 propionic acid, by Merck Group (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Hydrogen peroxide, by Merck Group (1 mentions) Streptomycin, by Merck Group (1 mentions) Penicillin, by Merck Group (1 mentions) Horseradish peroxidase, by Merck Group (1 mentions) Trolox, by Merck Group (1 mentions) Trizma base, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by ITW Reagents (1 mentions) Tyramine, by Merck Group (1 mentions) Acetylthiocholine iodide, by Merck Group (1 mentions) Tyrosinase, by Merck Group (1 mentions) Sodium chloride (nacl), by ITW Reagents (1 mentions) 4 aminoantipyrine, by Merck Group (1 mentions) 2 2 azobis 2 methylpropionamidine dihydrochloride, by Merck Group (1 mentions)

Spelling variants of this product

FeSO4 (148 citations) Ferrous sulfate (104 citations) Ferrous sulfate heptahydrate (FeSO4·7H2O) (12 citations) Ferrous sulfate heptahydrate (FeSO4) (4 citations)

11 protocols using ferrous sulfate feso4

1

Lipid Peroxidation Assay Protocol

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Caffeic acid phenethyl ester (CAPE), melatonin, ferrous sulfate (FeSO4) and hydrogen peroxide (H2O2) were purchased from Sigma-Aldrich (St. Louis, MO). The ALDetect™ Lipid Peroxidation Assay Kit for LPO was obtained from Enzo Life Science (Farmingdale, NY). All the used chemicals were of analytical grade and came from commercial sources.
Kokoszko-Bilska A., Stepniak J., Lewinski A, & Karbownik-Lewinska M. (2014). Protective antioxidative effects of caffeic acid phenethyl ester (CAPE) in the thyroid and the liver are similar to those caused by melatonin. Thyroid Research, 7, 5.
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2

Oxidative Stress Biomarker Quantification

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Melatonin, indole-3-propionic acid, ferrous sulfate (FeSO4) and hydrogen peroxide (H2O2) were purchased from Sigma (St. Louis, MO, USA). Ethanol (96%) was purchased from Stanlab (Lublin, Poland). The LPO-586 kit for LPO was obtained from Enzo Life Science (Farmingdale, NY, USA). All the used chemicals were of analytical grade and came from commercial sources.
Rynkowska A., Stępniak J, & Karbownik-Lewińska M. (2021). Melatonin and Indole-3-Propionic Acid Reduce Oxidative Damage to Membrane Lipids Induced by High Iron Concentrations in Porcine Skin. Membranes, 11(8), 571.
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3

Neuro-2a Cell Assays and Antioxidant Screening

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Neuro-2a (N2a) cell line was acquired from the Collection (ATCC, Manassas, VA, United States) whereas 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT), bovine serum albumin (BSA), penicillin, streptomycin, 2,2-diphenyl-1-picrylhydrazyl (DPPH), Trolox, Monoamine oxidase A (MAO-A), Trizma base, 4-aminoantipyrine, 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB), tyramine, levodopa (L-DOPA), horseradish peroxidase, 2,2′-azobis (2-methyl-propionamidine)-di-hydrochloride (AAPH), hydrogen peroxide (30%), vanillic acid, tyrosinase, 2,4,6-Tris (2-pyridyl)-1,3,5-triazine (TPTZ), galantamine, 2′,7′-Dichlorodihydrofluorescein Diacetate (DCFH-DA), xanthine, ferrous sulfate (FeSO4), acetylcholinesterase (AChE), acetylthiocholine iodide (ATCI) were obtained from Sigma-Aldrich (Madrid, Spain). Clorgyline and α-kojic were from Cymit química (Barcelona, Spain). Sodium carbonate (Na2CO3), HCl, NaCl, dimethyl sulfoxide (DMSO), methanol, and potassium phosphate were supplied from Panreac (Barcelona, Spain). Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), Nitroblue Tetrazolium (NBT) and xanthine oxidase were purchased from Vidrafoc (Barcelona, Spain) and iron chloride (FeCl3) from Laboaragon (Zaragoza, Spain). All reagents used were of analytical quality.
Cásedas G., Moliner C., Maggi F., Mazzara E, & López V. (2022). Evaluation of two different Cannabis sativa L. extracts as antioxidant and neuroprotective agents. Frontiers in Pharmacology, 13, 1009868.
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4

Antioxidant and Tyrosinase Inhibitory Assays

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2,2′-Azinobis 3-ethylbenzothiazoline-6-sulphonate (ABTS), 2,2-diphenyl-1-picrylhydrazyl-hydrate (DPPH), 2,4,6-tripyridyl-s-triazine (TPTZ), 6-hydroxy-2,5,7,8-tetramethyl chroman-2-carboxylic acid (Trolox), ammonium thiocyanate (NH4SCN), ferrous chloride (FeCl2), kojic acid, L-tyrosine, linoleic acid, oleanolic acid, ferrous sulfate (FeSO4), potassium persulfate (K2S2O8), sodium acetate trihydrate (CH3COONa.3H2O), sodium chloride (NaCl), sodium sulfate anhydrous (Na2SO4), and tyrosinase from mushroom (EC 1.14.18.1) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Acetic acid, hydrochloric acid, ethanol, calcium chloride (CaCl2), chloroform, de-ionized (DI) water, dimethyl sulfoxide (DMSO), glacial Acetic acid, petroleum ether, and sulfuric acid (H2SO4) were purchased from RCI Labscan Co., Ltd. (Bangkok, Thailand).
Prommaban A., Kheawfu K., Chittasupho C., Sirilun S., Hemsuwimon K, & Chaiyana W. (2022). Phytochemical, Antioxidant, Antihyaluronidase, Antityrosinase, and Antimicrobial Properties of Nicotiana tabacum L. Leaf Extracts. Evidence-based Complementary and Alternative Medicine : eCAM, 2022, 5761764.
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5

Lipid Peroxidation Assay Protocol

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17β-Estradiol, ferrous sulfate (FeSO4), and hydrogen peroxide (H2O2) were purchased from Sigma (St. Louis, MO, USA). Ethanol (96%) was purchased from Stanlab (Lublin, Poland). The LPO-586 kit for lipid peroxidation was obtained from Enzo Life Science (Farmingdale, NY, USA). All the used chemicals were of analytical grade and came from commercial sources.
Rynkowska A., Stępniak J, & Karbownik-Lewińska M. (2020). Fenton Reaction-Induced Oxidative Damage to Membrane Lipids and Protective Effects of 17β-Estradiol in Porcine Ovary and Thyroid Homogenates. International Journal of Environmental Research and Public Health, 17(18), 6841.
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6

Antioxidant Capacity Determination via FRAP Assay

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Methanol (CH3OH, CAS No. 67-56-1, 99.9% purity), 2,3,5-triphenyltetrazolium chloride or TPTZ (C19H15ClN4, CAS No. 298-96-4, 98% purity), hydrochloric acid (HCl, CAS No. 7647-01-0), ferric chloride (FeCl3, CAS No. 7705-08-0, 97% purity), ferrous sulfate (FeSO4, CAS No. 7782-63-0, 99% purity) and Folin–Ciocalteu reagent were purchased from Sigma-Aldrich (St. Louis, MO, USA). Gallic acid (CAS No. 5995-86-8, 98% purity) and urethane or ethyl carbamate (CAS No. 51-79-6, 98% purity) were purchased from Tokyo Chemical Industry (Tokyo, Japan).
Inthachat W., Suttisansanee U., Kruawan K., On-Nom N., Chupeerach C, & Temviriyanukul P. (2022). Evaluation of Mutagenicity and Anti-Mutagenicity of Various Bean Milks Using Drosophila with High Bioactivation. Foods, 11(19), 3090.
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7

Inulin and GOS Stock Solutions

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Inulin was purchased from Sigma Aldrich (Missouri, USA) and GOS from Carbosynth (Compton, UK), and were prepared as stock solutions of 80 mg/mL. Ferrous sulfate (FeSO4) was purchased from Sigma Aldrich and 100 mM stock solutions were prepared.
Oliero M., Calvé A., Fragoso G., Cuisiniere T., Hajjar R., Dobrindt U, & Santos M.M. (2021). Oligosaccharides increase the genotoxic effect of colibactin produced by pks+ Escherichia coli strains. BMC Cancer, 21, 172.
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8

Reagents for Cellular and Biochemical Assays

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Bicinchoninic acid (BCA), D-glucose, Drabkin’s reagent, ferrous sulfate (FeSO4), fluorescein isothiocyanate-dextran (FITCDextran), formic acid, mannitol and PKH67 (green fluorescent cell linker) were all purchased from Sigma-Aldrich (St. Louis, MO, USA). Fetal bovine serum (FBS), L-glutamine, penicillin/streptomycin, amphotericin B and HAT (hypoxanthine/aminopterin/thymidine) were obtained from Pan Biotech (Aidenbach, Germany). 3-(2-Pyridyl)-5,6-diphenyl-1,2,4-triazine-4′,4′′-disulfonic acid sodium salt (ferrozine) and 2,9-Dimethyl-1,10-phenanthroline(neocuproine) were purchased from Alfa Aesar (Kandel, Germany). MitoSOX Red mitochondrial superoxide indicator and pHrodo Red were purchased from Thermo Fisher (Carlsbad, CA, USA).
Turpin C., Apalama M.L., Carnero B., Otero-Cacho A., Munuzuri A.P., Flores-Arias M.T., Vélia E., Meilhac O., Bourdon E., Álvarez E, & Rondeau P. (2022). Impact of Enhanced Phagocytosis of Glycated Erythrocytes on Human Endothelial Cell Functions. Cells, 11(14), 2200.
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9

Preparation of Inulin and GOS Stocks

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Inulin was purchased from Sigma Aldrich (Missouri, USA) and GOS from Carbosynth (Compton, UK), and were prepared as stock solutions of 80 mg/mL. Ferrous sulfate (FeSO 4 ) was purchased from Sigma Aldrich and 100 mM stock solutions were prepared.
Oliero M., Calvé A., Fragoso G., Cuisiniere T., Hajjar R., Dobrindt U., & Santos M.M. (2020). Inulin and Galacto-oligosaccharides Increase the Genotoxic Effect of Colibactin Produced by pks+ Escherichia Coli Strains.
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10

Antioxidant Properties of Sandalwood Oil

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Sandalwood oil used in the experiment was supplied by Namcheon Co., Ltd. in Seoul, South Korea. Ascorbic acid (AA), Folin-Ciocalteu reagent (FC reagent), DPPH, ABTS, potassium persulfate, ferrous sulfate (FeSO 4 ), anhydrous sodium phosphate monobasic, ferrous chloride (FeCl 2 ), pyrogallol, and ammonium thiocyanate were purchased from Sigma Chemical Co. (St. Louis, Missouri, USA). Ferric chloride (FeCl 3 ) and sodium hydroxide (NaOH) were obtained from Wako Pure Chemical Industries Ltd. (Tokyo, Japan). The pBR322 DNA and 6X DNA loading dyes were purchased from Fermentas Inc. (Glen Burnie, MD). The catalase assay kit was purchased from Cayman Chemical Company (Ann Arbor, MI, USA).
Lee Y.M., Kim I.S., & Lim B.O. (2021). Effect of sandalwood oil on inhibition of reactive oxygen species generation and lipopolysaccharideinduced inflammation through down-regulation of the nuclear factor-κB signaling pathways. ScienceAsia, 47(3), 277-277.
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