Gist t1 cells

Kasumi-1, SKNO-1 (JCRB Cell Bank, Osaka, Japan), HMC-1.1 (Merck Millipore, Darmstadt, Germany), HMC-1.2 and pt18 cells were cultured at 37 °C in RPMI1640 medium supplemented with 10% FCS, penicillin, streptomycin, glutamine (Pen/Strep/Gln), and reducing agents (0.5 mM monothioglycerol or 50 μM 2-mercaptoethanol). For expansion of SKNO-1, 10 ng/mL granulocyte macrophage colony-stimulating factor (GM-CSF, Peprotech, Rocky Hill, NJ) was used. GIST-T1 cells (Cosmo Bio, Tokyo, Japan) were cultured at 37 °C in DMEM supplemented with 10% FCS and Pen/Strep/Gln. All human cell lines were authenticated by Short Tandem Repeat analysis at JCRB Cell Bank (Osaka, Japan) and tested for Mycoplasma contamination with a MycoAlert Mycoplasma Detection Kit (Lonza, Basel, Switzerland).

GIST-T1 cells were commercially purchased (Cosmo Bio, Tokyo, Japan) and GIST882 cells were kindly provided by Dr. Hogeun Kim. The cells were cultured in DMEM medium (Thermo Scientific, Rockford, IL, USA) supplemented with 10% fetal bovine serum (FBS), 1% penicillin and streptomycin. Cells were maintained in a humidified atmosphere of 5% CO₂ in air at 37°C.