Breeding pairs from male and female C57BL/6N, DBA/2, and FVB/N mice were purchased from Charles River Laboratories (Sulzfeld, Germany) at the age of 8 weeks. Mice for the behavioral tests were expanded at the Interdisciplinary Neurobehavioral Core, University of Heidelberg. Mice were housed in groups of three per cage. All mice were housed with food and water ad libitum under a standard 12-h light/dark cycle (7:00 p.m.–7:00 a.m.) with a regulated ambient temperature of 22 °C and at a relative humidity of 40–50%. All procedures were conducted in strict compliance with national and international guidelines for the Care and Use of Laboratory Animals. Extreme care was taken to minimize suffering for the animals. Animal experiments were approved by the local governing body (Regierungspräsidium Karlsruhe, Germany G-100/16; G-103/16; G-105/16).
Dba 2
Manufactured by Charles River Laboratories
Sourced in China, Germany, Japan
The DBA/2 is a laboratory mouse strain developed by The Jackson Laboratory. It is a commonly used inbred mouse strain that serves as a model for various research applications. The DBA/2 strain exhibits specific genetic and physiological characteristics that make it useful for scientific investigations, though its specific applications should be determined by the research objectives and requirements of the individual study.
Automatically generated - may contain errors
Lab products found in correlation
C57bl 6, by Charles River Laboratories (6 mentions)
Balb c, by Charles River Laboratories (3 mentions)
Dimethyl sulphoxide, by Merck Group (2 mentions)
R 7050, by Santa Cruz Biotechnology (2 mentions)
High phosphate diet, by Altromin (2 mentions)
Etanercept, by Pfizer (2 mentions)
C57bl 6n, by Charles River Laboratories (2 mentions)
Fvb n, by Charles River Laboratories (1 mentions)
Dba 2ncrl, by Charles River Laboratories (1 mentions)
Cd 1 mice, by Charles River Laboratories (1 mentions)
129s6 mice, by Taconic Biosciences (1 mentions)
Swiss webster, by Charles River Laboratories (1 mentions)
C57bl 6j mice, by Jackson ImmunoResearch (1 mentions)
B6c3f1, by Charles River Laboratories (1 mentions)
Dba 1, by Charles River Laboratories (1 mentions)
C3h hej, by Charles River Laboratories (1 mentions)
Balb c mice, by Corning (1 mentions)
C57bl 6 mice, by Corning (1 mentions)
C57bl 6j, by Charles River Laboratories (1 mentions)
Icr mice, by Charles River Laboratories (1 mentions)
16 protocols using dba 2
1
Breeding and Housing Procedures for Mouse Behavioral Studies
2
Murine Model of Vascular Calcification
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Female 8-week-old dilute-brown agouti 2 (DBA/2NCrl, hereafter referred to as DBA/2) mice were obtained from Charles River (Sulzfeld, Germany) and housed in a virus/antibody-free environment. These mice have an inherent susceptibility to high-phosphate diet-triggered calcification [15 (link), 16 (link)]. To induce media calcification, they were placed on high-phosphate diet (Altromin, Germany) containing 20.2 g phosphorus, 9.4 g calcium, 0.7 g magnesium and 500 IU vitamin D3 per kg. The standard chow contained 7.0 g phosphorus, 10.0 g calcium, 2.2 g magnesium and 1000 IU vitamin D3 per kg. Mice were then followed for 5–14 days and culled under anaesthesia. For the interventional studies, DBA/2 mice were divided into three treatment groups to receive vehicle control (dimethylsulphoxide; Sigma, St. Louis, MO), TNFα inhibitor etanercept (Pfizer, New York, NY) at a dose of 10 mg/kg body weight, or TNFα receptor antagonist R-7050 (Santa Cruz, Dallas, TX) at a dose of 6 mg/kg body weight, respectively [17 (link)]. These drugs were applied via intraperitoneal injections every alternate day. All animal experiments were approved by Austrian veterinary authorities (BMWF-66.010/0047-II/3b/2012) and corresponded to directive 2010/63/EU of the European Parliament.
3
Generation of F1 Hybrids and Chimera Mice
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TgOG2 was a generous gift from Dr. Jeff R. Mann, University of Melbourne, Victoria, Australia (Szabo et al., 2002 (link)). B6.Cg-Tg(CAG-GFP)Smoc mice were obtained from Shanghai Model Organisms Center (China). ICR, 129, C57BL/6, and DBA2 mice were purchased from Charles River (China). All mice were housed in a specific pathogen-free (SPF) animal facility at Tongji University, Shanghai, China. F1 hybrids between B6.Cg-Tg(CAG-GFP)Smoc (male) and 129 (female), and F1 hybrids between TgOG2 (male) and 129 (female) mice were generated with 7- to 8-week-old females. ICR females aged 6–8 weeks were used as donors of tetraploid embryos and chimera embryos. ICR adult females aged 8–10 weeks were used as pseudopregnant recipients. All animal experiments and breeding procedures were performed in accordance with the experimental animal use guide of Tongji University.
4
Renal Calcification Model in DBA/2 Mice
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Female 8-week-old dilute brown non-agouti 2 (DBA/2NCrl, hereafter referred to as DBA/2) mice were obtained from Charles River (Sulzfeld, Germany) and housed in a virus/antibody-free environment in the laboratory animal facility of the Medical University of Graz. These mice are susceptible to ectopic renal calcification and media calcification when exposed to increased oral phosphate loads [14 (link)–16 (link)]. In order to cause renal damage, these mice were fed standard chow (SCD; n = 8) or high-phosphate diet for 4 (HPD4; n = 4) or 7 (HPD7; n = 4) days with subsequent return to SCD until day 84 after starting HPD diet. The high-phosphate diet (Altromin, Lage, Germany) contained 20.2 g of phosphorus, 9.4 g of calcium, 0.7 g of magnesium, and 500 IU/kg of vitamin D3. The standard chow contained 7.0 g of phosphorous, 10.0 g of calcium, 2.2 g of magnesium, and 1000 IU/kg of vitamin D3.
All animal experiments were approved by the Committee of the Ethics of Animal Experiments of the Austrian Ministry (BMWFW-66.010/0061-WF/V/3b/2016). All experiments were conducted under strict adherence of the law of Austria.
All animal experiments were approved by the Committee of the Ethics of Animal Experiments of the Austrian Ministry (BMWFW-66.010/0061-WF/V/3b/2016). All experiments were conducted under strict adherence of the law of Austria.
5
DBA/2 Mouse Model of Vascular Calcification
6
Mouse Experiments for Psychiatric Research
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The animal experiments were approved by local authorities and conducted according to current regulations for animal experimentation in Germany and the European Union (European Communities Council Directive 2010/63/EU). Three-week-old, male B6 mice (n = 15, C57BL/6N, Martinsried, Germany) and DBA mice (n = 15, DBA/2, Charles River, Sulzfeld, Germany) were purchased and housed in groups of three for 11 weeks at the animal facility of the Max Planck Institute of Psychiatry under standard conditions as previously described [35 (link)].
7
Murine Housing and Breeding Protocols
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Mice were housed in accordance with NIH guidelines in Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC)-accredited facilities at the Salk Institute for Biological Studies. The Institutional Animal Care and Use Committee at the Salk Institute approved all animal studies. C57BL/6J mice were either purchased from The Jackson Laboratory or bred in-house. Eight-weeks-old DBA/2, Swiss Webster, BALB/c, and FVB mice were purchased from Charles River Laboratories. CD-1 mice were either purchased from Charles River Laboratories or bred in-house. Eight-weeks-old 129S6 mice were purchased from Taconic Biosciences. Ptf1aCreERTM/+ and RosaYFP strains have been previously described and were purchased from The Jackson Laboratory (Pan et al., 2013 (link)). FLARE25 (Il25F25/F25) mice (C57Bl/6J strain) were generously provided by the Locksley (University of California at San Francisco, CA, United States) and von Moltke (University of Washington, WA, United States) laboratories and were bred to the CD-1 mouse strain (von Moltke et al., 2016 (link)). F1 mice were used for experiments.
8
Mouse Husbandry and Acclimation
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Female 5- to 8-week-old C3H/He, C57BL/6, B6C3F1 (C57BL/6 × C3H/He), BALB/c, and DBA/2 mice were obtained from Charles River Japan (Kanagawa, Japan) and CLEA Japan (Tokyo, Japan). All animals were allowed to acclimatize and recover from shipping-related stress for 1 week prior to the study. The animals were allowed free access to chlorinated water and irradiated food, and were kept in a controlled light–dark cycle (12–12 h)32 .
9
Mouse Strains for Immunological Studies
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Tlr7−/− and Tlr9−/− mice described previously (48 (link), 49 (link)) were kindly provided by S. Akira, Osaka University, Osaka, Japan. C57BL/6 and BALB/c mice were purchased from CLEA Japan. DBA/1, DBA/2, and B6D2F1 (BDF1) mice were obtained from Charles River Laboratories Japan. BXSB/MpJ-Yaa and BXSB/MpJ-Yaa+ mice were purchased from Japan SLC. CAG-Cre transgenic mice were obtained from The Jackson Laboratory. All animal experiments were done in accordance with guidelines of the University of Tokyo and RIKEN Kobe Branch.
10
Mouse Strain Procurement and Housing
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All mice were housed at the laboratory animal facility that had been accredited by AAALAC (Association for Assessment and Accreditation of Laboratory Animal Care International) and the IACUC (Institutional Animal Care and Use Committee) of Tsinghua University. Mouse animal protocols were approved by the laboratory animal facility. C57BL/6, C3H/HEJ, and DBA/2 mice were purchased from Charles River. We obtained B6.SJL-PtprcaPepcb/BoyJ mice from Dr. Li Wu’s Lab (School of Life Science, Tsinghua University).
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