Using Elshire et al.’s (2011) (link) methodology with modifications, GBS data were generated. In brief, each 200 ng of DNA sample was digested with five units of the ApeK1 enzyme at 75°C for 4 h and cooled down to 4°C, followed by GBS library preparation. Unique barcodes and a “common” adapter were used for each individual ligation. After ligation, they were pooled and purified using AMpure DNA purification beads and the genomic fragments were then amplified in 50-µl volumes. GBS library purification was carried out using Agencourt AMPure XP SPRI magnetic beads (Beckman Coulter). The quality of the GBS library was assessed using the 4150 TapeStation System (Catalog: G2992AA, Agilent). The GBS library was sequenced using the Illumina HiSeq 2000 platform with paired-end (PE) reads of 150 × 2 bp.
Agencourt ampure xp spri magnetic beads
Manufactured by Beckman Coulter
The Agencourt AMPure XP SPRI magnetic beads are a sample preparation tool used in various molecular biology applications. They facilitate the purification and size-selection of nucleic acids, such as DNA and RNA, from complex sample matrices. The beads utilize Solid Phase Reversible Immobilization (SPRI) technology to selectively bind and separate target molecules, enabling efficient cleanup and concentration of samples.
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Lab products found in correlation
2 protocols using agencourt ampure xp spri magnetic beads
1
Genotyping-By-Sequencing Library Preparation
2
DNA Purification Using AMPure XP SPRI Beads
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Water was added prior to each purification reaction to reduce liquid handling errors during the purification step: 10 µL or 70 µL for manual and automatic purification, respectively. A 1× volume of Agencourt AMPure XP SPRI magnetic beads (Beckman Coulter) was used to purify the sample from residual enzyme, nucleotides, and primer dimers traces, according to the recommended protocol. This process was done either manually on a DynaMag-96 Side Skirted Magnet (Life Technologies) or automatically, using a robot (Evoware, Tecan) (Supplemental Note S2 ) and a Magnum FLX magnetic plate (Alpaqua).
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