Anti nf kb p65 c 20 sc 372

A monoclonal antibody against E-selectin (clone 7A9) was obtained from the American Type Culture Collection; anti-E-selectin (A-10: sc-137203) and anti-NF-kB p65 (C-20: sc-372) antibodies were obtained from Santa Cruz Biotechnology; anti-phospho-NF-κB p65 Ser 536 (#3031), anti-SAPK/JNK (#9252), anti-p38 MAPK (#9212), anti-phospho-SAPK/JNK (#9251), and anti-phospho-p38 MAPK (#4511) antibodies were obtained from Cell Signaling Technology; anti-lamin A/C (SAB4200236) and anti-actin (A5060) antibodies were obtained from Sigma-Aldrich; an anti-α-tubulin antibody (PM054-7) was obtained from Medical & Biological Laboratories; an anti-acetyl-histone H3 antibody (06-599) was obtained from Millipore; an Alexa Fluor 488-conjugated goat anti-mouse IgG antibody (A11017) was obtained from Life Technologies; horseradish peroxidase (HRP)-linked anti-mouse (NA931V) and anti-rabbit (NA9340V) secondary antibodies were obtained from GE Healthcare.

Western blot and immunostaining were performed using standard protocols [26 (link)]. Anti-LOX-1 (LOX19-22) sc-66155, anti-AR (N-20) sc-816, anti-GAPDH (0411) sc-47724, anti-NF-kB p65 C-20) sc-372 and anti-IκB-alfa (C-21) sc-371, and anti-STAT3 (c-20) sc-482 antibodies were obtained from Santa Cruz Biotechnology. Anti-p-p65 s536 7F1, anti-p-IκB-alfa S32/S36 5A5, androgen receptor (AR-V7 Specific), and anti-p-STAT3 (Y705) (D3A7) antibodies were obtained from Cell Signaling. For Western blot, the anti-mouse IgG-Alexa Fluor 680 and anti-rabbit IgG-Alexa Fluor 790 secondary antibodies were obtained from Jackson ImmunoResearch. Western blots were analyzed using LICOR-CLX. An anti-rabbit IgG/FITC secondary antibody from Jackson ImmunoResearch was used for immunofluorescences. Photographs were obtained by confocal microscopy (Olympus IX81, Japan). To determine IL-6 concentration from supernatants, the human ELISA Kit (D6050, R&D) was used according to manufacturer’s instructions.