Cryostat sections (4μm) of frozen kidneys were fixed with ice-cold acetone for 10min, and blocked with 10% goat serum to decrease background staining. FITC-conjugated goat anti-mouse C3 Ab (4.0 mg/ml, MP Biomedicals) was used directly at 1:500 dilution. Under ×200 magnification, 10 viewing fields from sections of each animal were photographed. Areas of positive staining were highlighted and the fluorescence intensity of C3 was determined using the plugin “Measure particles” of ImageJ software. F4/80-positive mononuclear phagocytes were visualized by staining with a rat anti-mouse F4/80 Ab (1.0 mg/ml, AbD seroTEC) used at 1:50 dilution followed by Alexa Fluor 555-goat anti-rat IgG (2.0 mg/ml, Invitrogen) used at 1:2000 dilution. Under 400x magnification, F4/80+ cells were counted by examining ten viewing fields randomly selected from the outer medulla on each slide.
Fitc conjugated goat anti mouse c3 ab
Manufactured by MP Biomedicals
FITC-conjugated goat anti-mouse C3 Ab is a labelled antibody that binds to the complement component C3 in mouse samples.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using fitc conjugated goat anti mouse c3 ab
1
Quantifying Kidney Complement C3 and Macrophages
2
Quantifying Kidney Complement C3 and Macrophages
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Cryostat sections (4μm) of frozen kidneys were fixed with ice-cold acetone for 10min, and blocked with 10% goat serum to decrease background staining. FITC-conjugated goat anti-mouse C3 Ab (4.0 mg/ml, MP Biomedicals) was used directly at 1:500 dilution. Under ×200 magnification, 10 viewing fields from sections of each animal were photographed. Areas of positive staining were highlighted and the fluorescence intensity of C3 was determined using the plugin “Measure particles” of ImageJ software. F4/80-positive mononuclear phagocytes were visualized by staining with a rat anti-mouse F4/80 Ab (1.0 mg/ml, AbD seroTEC) used at 1:50 dilution followed by Alexa Fluor 555-goat anti-rat IgG (2.0 mg/ml, Invitrogen) used at 1:2000 dilution. Under 400x magnification, F4/80+ cells were counted by examining ten viewing fields randomly selected from the outer medulla on each slide.
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