Horseradish peroxidase hrp conjugated secondary antibodies against mouse and rabbit igg

Antibody against CDC42 was purchased from Cytoskeleton, Inc. (Denver, CO) and Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Antibody against AKT2, AKT, survivin, N-myc, cleaved caspase-3, cleaved PARP and cell lysis buffer were obtained from Cell Signaling Technology (Beverly, MA). Twist antibody and horseradish peroxidase (HRP)-conjugated secondary antibodies against mouse and rabbit IgG were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Anti β-actin monoclonal antibody and fetal bovine serum (FBS) were from Sigma-Aldrich (St. Louis, MO). NuPAGE Novex 4–12% Bis–Tris Gel and Lipofectamine 2000 were purchased from Invitrogen (Carlsbad, CA). Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Molecular Technologies, Inc. (Santa Clara, CA). Agarose (SeaPlaque®) was from Lonza Inc. (Allendale, NJ). Cell Death Detection ELISA^Plus was purchased from Roche Applied Science (Indianapolis, IN).

Primary antibodies against AURKA and cell lysis buffer were obtained from Cell Signaling Technology (Beverly, MA, USA); focal adhesion kinase (FAK) and phosphorylated (p)-FAK from BD Biosciences (San Jose, CA, USA). Antibody against β-actin, fetal bovine serum (FBS) and cobalt (II) chloride hexahydrate were from Sigma (St. Louis, MO, USA). NuPAGE Novex 4–12% Bis–Tris Gel and Lipofectamine 2000 were purchased from Invitrogen (Carlsbad, CA, USA). Horseradish peroxidase (HRP)-conjugated secondary antibodies against mouse and rabbit IgG were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Chemiluminescence (ECL) HRP substrate were purchased from Millipore (Billerica, MA, USA) and Perkin Elmer (Alameda, CA, USA). MLN8237 was from Selleckchem (Houston, TX, USA).