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Anti trop2

Manufactured by R&D Systems
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Anti-Trop2 is a protein product designed for use in laboratory research. It functions as an antibody that specifically binds to the Trop2 protein.

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Lab products found in correlation

Anti phospho jnk, by Abcam (1 mentions) Anti p38, by Abcam (1 mentions) Anti jnk, by Abcam (1 mentions) Anti phospho p38, by Abcam (1 mentions) Anti α tubulin, by Merck Group (1 mentions) Anti phospho erk1 2, by Cell Signaling Technology (1 mentions) Anti erk1 2, by Cell Signaling Technology (1 mentions) Bond max, by Leica (1 mentions) Bond polymer refine detection kit, by Leica (1 mentions) Pyrrole, by Merck Group (1 mentions) Anti n cadherin, by R&D Systems (1 mentions) Biotinylated anti cd9, by Abcam (1 mentions) Anti egfr, by R&D Systems (1 mentions) Biotinylated anti epcam, by R&D Systems (1 mentions) Anti vimentin, by R&D Systems (1 mentions) Sodium hydroxide (naoh), by Merck Group (1 mentions) N 3 dimethylaminopropyl n ethylcarbodiimide hydrochloride edc, by Merck Group (1 mentions) N hydroxysuccinimide, by Merck Group (1 mentions)

4 protocols using anti trop2

1

Immunohistochemical Analysis of Thyroid Lesions

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Immunohistochemistry (IHC) analysis was performed to study altered protein expression in formalin-fixed and paraffin-embedded human thyroid lesions. The following primary antibodies were used: anti-Trop2 (R&D Systems, Minneapolis, MN), and anti-MMP2 (Lifespan Bioscience, Seattle). The degree of immunostaining was examined and scored independently by two observers by combining both the proportion of positively staining tumor cells and the staining intensity as previously described [23 (link)].
Guan H., Guo Z., Liang W., Li H., Wei G., Xu L., Xiao H, & Li Y. (2017). Trop2 enhances invasion of thyroid cancer by inducing MMP2 through ERK and JNK pathways. BMC Cancer, 17, 486.
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2

Quantifying Protein Expression via Western Blotting

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Western blotting (WB) was performed according to a standard method as described previously [25 (link)]. The following primary antibodies were used: anti-Trop2 (R&D Systems, Minneapolis, MN), anti-ERK1/2 and anti-phospho-ERK1/2 (Cell Signaling Technology, Beverly, MA), anti-p38, anti-phospho-p38, anti-JNK, and anti-phospho-JNK (Abcam, Cambridge, MA), and anti-α-tubulin (Sigma-Aldrich, St. Louis, MO).
Guan H., Guo Z., Liang W., Li H., Wei G., Xu L., Xiao H, & Li Y. (2017). Trop2 enhances invasion of thyroid cancer by inducing MMP2 through ERK and JNK pathways. BMC Cancer, 17, 486.
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3

Trop2 Immunohistochemistry Protocol

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Immunohistochemistry was conducted on a 4-μm tissue array section using a BOND-MAX automated immunostainer and a Bond Polymer Refine Detection kit (Leica Microsystems, Wetzlar, Germany) according to the manufacturer’s instructions. The primary antibody used was anti-Trop2 (R&D Systems, Minneapolis, MN, USA; 1:800). The expression of Trop2 was determined by examining the cell membrane and the intensity and percentage of cells expressing Trop2 were evaluated. Histology index (H index) was calculated by multiplying the intensity score (0 = negative; 1 = weak; 2 = moderate; 3 = strong) and percentage of positive cells (range = 0–100), to obtain a score range from 0 to 300.
Riera K.M., Jang B., Min J., Roland J.T., Yang Q., Fesmire W.T., Camilleri-Broet S., Ferri L., Kim W.H., Choi E, & Goldenring J.R. (2020). Trop2 is Upregulated in the Transition to Dysplasia in the Metaplastic Gastric Mucosa. The Journal of pathology, 251(3), 336-347.
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4

Enrichment and Detection of Extracellular Vesicles

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Pyrrole, poly(sodium 4-styrenesulfonate) (PSS), N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS), streptavidin, and sodium hydroxide (NaOH) were obtained from Sigma Aldrich (St. Louis, MO, USA). Anodized aluminum oxide (AAO) membrane filter (pore diameter, 200 nm) was purchased from Whatman (Pittsburgh, PA, USA). Silver paste (P-100 type) was obtained from CANS. NHS-SS-biotin was purchased from CovaChem (Loves Park, Illinois, USA). Biotinylated anti-EpCAM, anti-EGFR, anti-vimentin, anti-N-cadherin, and anti-Trop2 were obtained from R&D system (Minneapolis, MN, USA). Biotinylated anti-CD63 and anti-CD81 were purchased from AnCell (Oak Park, Minnesota, USA). Biotinylated anti-CD9 was obtained from Abcam (Cambridge, UK).
Lim J., Choi M., Lee H., Han J.Y, & Cho Y. (2019). A Novel Multifunctional Nanowire Platform for Highly Efficient Isolation and Analysis of Circulating Tumor-Specific Markers. Frontiers in Chemistry, 6, 664.
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