PP monofilament was obtained from Shandong Xinhua Medical Device Co., LTD, China. Poly(ε-caprolactone) (PCL, Mn = 80 000), phosphate buffered saline (PBS) tablets, glutaraldehyde and paraformaldehyde were ordered from Sigma-Aldrich (St. Louis, MO, USA). Hexafluoro-2-propanol (HFIP) was acquired from Oakwood Inc., West Columbia, SC, USA. Hank's balanced salt solution (HBSS) was obtained from Gibco, USA. Human dermal fibroblasts (HDFs) were purchased from ScienCell Research Laboratories, Inc., USA. Fibroblast Medium was obtained from Biomaterials USA (Catalog # FC-10 × 50 mL, Richmond, VA, USA). 1,1′-Dioctadecyl-3,3,3′3′-tetramethylindocarbocyanine perchlorate (DiI), LIVE/DEAD™ Reduced Biohazard Cell Viability Kit, AlexaFluor 488/546 Phalloidin and 4′-6-diamidino-2-phenylindole (DAPI) were purchased from ThermoFisher (Carlsbad, CA, USA). Alamar Blue™ reagent was obtained from VWR, USA.
Alexafluor 488 546 phalloidin
Manufactured by Thermo Fisher Scientific
Sourced in United States
AlexaFluor 488/546 Phalloidin is a fluorescent dye used for staining and visualizing filamentous actin (F-actin) in cells. It binds tightly and specifically to F-actin, allowing for the detection and localization of the actin cytoskeleton.
Automatically generated - may contain errors
Lab products found in correlation
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (4 mentions)
Phosphate buffered saline (pbs), by Merck Group (2 mentions)
Paraformaldehyde (pfa), by Merck Group (2 mentions)
Glutaraldehyde, by Merck Group (2 mentions)
Poly ε caprolactone, by Merck Group (1 mentions)
Hanks balanced salt solution (hbss), by Thermo Fisher Scientific (1 mentions)
Polycaprolactone, by Merck Group (1 mentions)
Penicillin streptomycin fungizone, by Merck Group (1 mentions)
Hank s balanced salt solution, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Alamarblue reagent, by Bio-Rad (1 mentions)
2 protocols using alexafluor 488 546 phalloidin
1
Fibroblast Culturing on PCL Scaffolds
2
PCL Scaffold Fabrication for Retinal Cell Culture
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Poly(Ɛ-caprolactone) (PCL, Mn = 80,000), glutaraldehyde, dimethyl sulfoxide (DMSO), phosphate-buffered saline (PBS), Hank’s balanced salt solution (HBSS), antibiotics (penicillin–streptomycin–fungizone), and paraformaldehyde were ordered from Sigma-Aldrich (St. Louis, MO, USA). Hexafluoro-2-propanol (HFIP) was acquired from Oakwood Inc. (West Columbia, SC, USA). Thiolated a5b1 integrin-binding peptides, CellPhilic™ xeno-free and serum-free culture medium for human epithelial cells (SKU: ESC-10×50 mL), vinylsulfone-functionalized polyvinyl alcohol (PVA-VS), and 4-arm polyethylene glycol acrylate (PEGTA) were ordered from Biomaterials USA LLC (Richmond, VA, USA). LIVE/DEAD™ Reduced Biohazard Cell Viability Kit, AlexaFluor 488/546 Phalloidin, and 4′-6-diamidino-2-phenylindole (DAPI) were purchased from ThermoFisher (Carlsbad, CA, USA). Alamar Blue™ reagent was obtained from Bio-Rad Laboratories (Hercules, CA, USA). The adult human retinal pigmented epithelium-19 (ARPE-19, ATCC-CRL-2302™) cell line was purchased from American Type Culture Collection (Manassas, VA, USA).
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